Van der Kraak G, Rosenblum P M, Peter R E
Department of Zoology, University of Guelph, Ontario, Canada.
Gen Comp Endocrinol. 1990 Aug;79(2):233-9. doi: 10.1016/0016-6480(90)90108-x.
The possible involvement of growth hormone (GH) in the regulation of ovarian function in the goldfish was investigated by determining the effects of common carp GH on steroid production by vitellogenic and preovulatory ovarian follicles incubated in vitro. Carp GH acts in a dose-dependent manner to potentiate the actions of common carp gonadotropin (GtH) on the production of 17 beta-estradiol and testosterone by vitellogenic ovarian follicles and the actions of human chorionic gonadotropin (hCG) on testosterone production by preovulatory ovarian follicles. Carp GH alone had no effect on basal steroid secretion by either class of ovarian follicles. Chum salmon GH but not bovine GH also enhanced carp GtH-induced production of 17 beta-estradiol by vitellogenic ovarian follicles. Common carp prolactin had no effects on basal or GtH-stimulated steroid production by vitellogenic or preovulatory ovarian follicles. The actions of carp GH on preovulatory follicles were not apparent when tested with the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, suggesting that GH may act to enhance either the formation or actions of cAMP. In summary, these data demonstrate that GH has a direct modulatory effect on GtH-stimulated steroid production and suggest that GH may be an important regulator of follicular development in the goldfish.
通过测定鲤生长激素（GH）对体外培养的卵黄生成期和排卵前卵巢卵泡类固醇生成的影响，研究了生长激素在金鱼卵巢功能调节中的可能作用。鲤GH以剂量依赖的方式增强鲤促性腺激素（GtH）对卵黄生成期卵巢卵泡17β-雌二醇和睾酮生成的作用，以及人绒毛膜促性腺激素（hCG）对排卵前卵巢卵泡睾酮生成的作用。单独的鲤GH对这两类卵巢卵泡的基础类固醇分泌均无影响。大麻哈鱼GH而非牛GH也增强了鲤GtH诱导的卵黄生成期卵巢卵泡17β-雌二醇的生成。鲤催乳素对卵黄生成期或排卵前卵巢卵泡的基础或GtH刺激的类固醇生成均无影响。用磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤进行测试时，鲤GH对排卵前卵泡的作用不明显，这表明GH可能通过增强cAMP的形成或作用来发挥作用。总之，这些数据表明GH对GtH刺激的类固醇生成具有直接调节作用，并提示GH可能是金鱼卵泡发育的重要调节因子。
