Gellermann Gerald P, Appel Thomas R, Davies Peter, Diekmann Stephan
Leibniz Institute for Age Research, Fritz-Lipmann-Institut, Beutenbergstrasse 11, D-07745 Jena, Germany.
Biol Chem. 2006 Sep;387(9):1267-74. doi: 10.1515/BC.2006.157.
By using qualitative and quantitative high-performance thin layer chromatography (hpTLC) we found lipids associated with purified Alzheimer's (AD) paired helical filaments (PHF) in an amount of 1.4+/-0.2% of the total anhydrous mass. Compared to normal brain tissue these lipids have an unusual lipid class composition. The most prominent lipid classes were phosphatidylcholine (PC), cholesterol (CH), galactocerebrosides (GC) and sphingomyelin (SM). In addition, the use of micro high-performance liquid chromatography (HPLC) in combination with matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) allowed the determination of the molecular species of the polar membrane lipid classes present in PHF. The lipid pattern of intracellular PHF shows many characteristics of the conserved lipid pattern previously described for extracellular amyloid fibrils, suggesting similarities in their pathway of formation.
通过使用定性和定量高效薄层色谱法（hpTLC），我们发现与纯化的阿尔茨海默病（AD）双螺旋丝（PHF）相关的脂质占总无水质量的1.4±0.2%。与正常脑组织相比，这些脂质具有不同寻常的脂质类别组成。最主要的脂质类别是磷脂酰胆碱（PC）、胆固醇（CH）、半乳糖脑苷脂（GC）和鞘磷脂（SM）。此外，将微型高效液相色谱法（HPLC）与基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF-MS）结合使用，能够测定PHF中存在的极性膜脂质类别的分子种类。细胞内PHF的脂质模式显示出许多先前描述的细胞外淀粉样纤维保守脂质模式的特征，表明它们在形成途径上具有相似性。
