Culpan Doris, Prince Jonathan A, Matthews Sonia, Palmer Laura, Hughes Anthony, Love Seth, Kehoe Patrick G, Wilcock Gordon K
Dementia Research Group, Institute of Clinical Neurosciences, Department of Clinical Sciences at North Bristol, University of Bristol, John James Buildings, Frenchay Hospital, Frenchay, Bristol, BS16 1LE, United Kingdom.
Neurosci Lett. 2006 Nov 13;408(2):141-5. doi: 10.1016/j.neulet.2006.08.068. Epub 2006 Sep 14.
Interleukin 10 (IL-10) is an important anti-inflammatory cytokine produced in response to neuroinflammation and might be involved in modulating the progression of Alzheimer's disease (AD) through inhibiting the action of pro-inflammatory cytokines. We have used immunohistochemistry, Western blotting, real time-PCR (RT-PCR) on frontal (BA 6/24) and temporal (BA 20-22) neocortex and hippocampus from AD and control brains as well as genetic association analysis to address the possible involvement of IL-10 in AD. Expression of IL-10 in AD and control brains at both protein and mRNA levels were detected. However, the level of expression, particularly of IL-10 protein, varied considerably in individual brains and we did not find a significant difference between AD and controls. Using direct sequencing we examined five single nucleotide polymorphisms (SNPs) (-3538, -1354, -1087, -824, -597) and two microsatellites (IL-10-G, IL-10-R) in the promoter region of the IL-10 gene. None of the identified SNPs were found to be associated with AD either individually or as haplotypes. Levels of IL-10 protein and gene expression examined also did not appear to be related to AD. Despite this being a relatively small sample, these data suggest that IL-10 does not play a major role in the development of AD.
白细胞介素10（IL-10）是一种在神经炎症反应中产生的重要抗炎细胞因子，可能通过抑制促炎细胞因子的作用参与调节阿尔茨海默病（AD）的进展。我们运用免疫组织化学、蛋白质印迹法、实时定量聚合酶链反应（RT-PCR）对AD患者及对照者大脑的额叶（BA 6/24）、颞叶（BA 20-22）新皮质和海马进行检测，并开展基因关联分析，以探讨IL-10在AD中的可能作用。检测了AD患者及对照者大脑中IL-10在蛋白质和mRNA水平的表达。然而，IL-10的表达水平，尤其是蛋白质水平，在个体大脑中差异很大，我们未发现AD患者与对照者之间存在显著差异。我们采用直接测序法检测了IL-10基因启动子区域的五个单核苷酸多态性（SNP）（-3538、-1354、-1087、-824、-597）和两个微卫星（IL-10-G、IL-10-R）。所鉴定的SNP无论是单个还是作为单倍型，均未发现与AD相关。所检测的IL-10蛋白质水平和基因表达水平似乎也与AD无关。尽管样本量相对较小，但这些数据表明IL-10在AD的发生发展中并不起主要作用。
