Mathai Jaisy, Resmi K R, Sulochana P V, Sathyabhama S, Baby Saritha G, Krishnan Lissy K
Division of Blood Transfusion Services, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram-695011, Kerala, India.
Platelets. 2006 Sep;17(6):393-6. doi: 10.1080/09537100600757695.
Platelet discoid shape is known to correlate with in vivo viability after transfusion. Measurement of shape change requires invasive sampling and laborious assays, which is difficult to perform in a blood transfusion center as a routine test for quality control of stored platelets. The objective of this study was to establish suitability of swirling assessment in stored platelet suspension as a routine test for quality check, by comparing platelet shape change measurement carried out in parallel. The study was done in two types of bags obtained from different manufactures (Groups 1 and 2). Platelet concentrates (PC) were stored for 120 h and samples drawn at 24-h intervals, optical analysis at 540 nm was carried out to quantify shape change in response to an agonist adenosine diphosphate (ADP). The same bags were subjected to swirling assessment, by two blood bank personnel independently and graded as positive (+, ++, +++) or as negative, based on the silky appearance of the suspension. Swirling negative platelets were prepared by storing platelets at 4 degrees C for 24 h and were compared with swirling positive platelets. Other parameters studied in the samples drawn at 24-h intervals were platelet count, mean platelet volume, and blood gases. Swirling assessment results correlated well with shape change measurement at each study period with a P value significant at 0.02 and 0.04 for group 1 and 2 bags, respectively. In the negative swirling controls, extent of shape change was lower than the extent in test bags, showing reduced capacity to respond to ADP at 4 degrees C. The results of the study indicate that by simple swirling measurements, stored PC can be monitored for loss of discoid shape before they are transfused. Gas tension and pH were with in permissible limits. Therefore, inspection of swirling can be a reliable method of quality control as it correlates with platelet function. The platelets that retain the discoid shape in vitro at the time of transfusion are expected to be functional in vivo.
已知血小板的盘状形态与输血后的体内存活率相关。形状变化的测量需要进行侵入性采样和繁琐的检测，作为储存血小板质量控制的常规检测，在输血中心很难实施。本研究的目的是通过比较同时进行的血小板形状变化测量，确定在储存的血小板悬液中进行漩涡评估作为质量检查常规检测的适用性。该研究在从不同制造商获得的两种类型的袋子中进行（第1组和第2组）。血小板浓缩物（PC）储存120小时，每隔24小时抽取样本，在540nm处进行光学分析，以量化对激动剂二磷酸腺苷（ADP）的形状变化反应。同一袋子由两名血库工作人员独立进行漩涡评估，并根据悬液的丝滑外观评为阳性（+、++、+++）或阴性。通过将血小板在4℃下储存24小时制备漩涡阴性血小板，并与漩涡阳性血小板进行比较。在每隔24小时抽取的样本中研究的其他参数包括血小板计数、平均血小板体积和血气。在每个研究阶段，漩涡评估结果与形状变化测量结果相关性良好，第1组和第2组袋子的P值分别为0.02和0.04，具有显著性。在阴性漩涡对照中，形状变化程度低于测试袋中的程度，表明在4℃下对ADP的反应能力降低。研究结果表明，通过简单的漩涡测量，可以在储存的PC输血前监测其盘状形态的丧失。气体张力和pH值在允许范围内。因此，漩涡检查可以作为一种可靠的质量控制方法，因为它与血小板功能相关。预计在输血时体外保持盘状形态的血小板在体内具有功能。
