Departamento de Saúde, Faculdade de Medicina Veterinária, Centro Universitário Ritter dos Reis - UniRitter - Campus FAPA, Av. Manoel Elias, 2001, 91240-261, Porto Alegre, Brazil.
Departamento de Patologia Clínica Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
BMC Vet Res. 2020 Jan 30;16(1):25. doi: 10.1186/s12917-020-2254-5.
Platelets undergo structural, biochemical and functional alterations when stored, and platelet storage lesions reduce platelet function and half-life after transfusion. The objective of this study was to evaluate stored canine platelet concentrates with platelet aggregation, flow cytometry and biochemistry assays. Twenty-two bags of canine platelet concentrates were obtained by the platelet-rich plasma method and were assessed on days 1, 3 and 5 after collection. Parameters such as platelet counts, residual leukocytes, platelet swirling, glucose, lactate, pH, CD62P expression (platelet activation), JC-1 (mitochondrial function) and annexin V (apoptosis and cell death) were assessed.
Over the five days of storage there was a significant decrease in glucose, HCO, pCO, ATP, pH, swirling and mitochondrial function, associated with a significant increase in lactate levels and pO. At the end of storage pH was 5.9 ± 0.6 and lactate levels were 2.8 ± 1.2 mmol/L. Results of the quality parameters evaluated were similar to those reported in human platelets studies. The deleterious effects of storage were more pronounced in bags with higher platelet counts (> 7.49 × 10/unit), suggesting that canine platelet concentrates should not contain an excessive number of platelets.
Quality parameters of canine platelets under standard storage conditions were similar to those observed in human platelets. Our results have potential to be used for the routine evaluation and quality control in veterinary blood banks.
血小板在储存过程中会发生结构、生化和功能改变，血小板储存损伤会降低输注后的血小板功能和半衰期。本研究旨在通过血小板聚集、流式细胞术和生物化学检测来评估储存的犬血小板浓缩物。通过富血小板血浆法获得 22 袋犬血小板浓缩物，在采集后第 1、3 和 5 天进行评估。评估的参数包括血小板计数、残留白细胞、血小板旋转、葡萄糖、乳酸、pH 值、CD62P 表达（血小板活化）、JC-1（线粒体功能）和 Annexin V（凋亡和细胞死亡）。
在储存的 5 天内，葡萄糖、HCO、pCO、ATP、pH 值、旋转和线粒体功能显著下降，而乳酸水平显著升高。储存结束时 pH 值为 5.9±0.6，乳酸水平为 2.8±1.2mmol/L。评估的质量参数结果与人类血小板研究报告的结果相似。血小板计数较高（>7.49×10/单位）的袋中储存的有害影响更为明显，这表明犬血小板浓缩物不应含有过多的血小板。
在标准储存条件下，犬血小板的质量参数与人类血小板观察到的相似。我们的研究结果有可能用于兽医血库的常规评估和质量控制。
