Suh Jae Mi, Yu Cheng-Tai, Tang Ke, Tanaka Toshiya, Kodama Tatsuhiko, Tsai Ming-Jer, Tsai Sophia Y
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
Mol Endocrinol. 2006 Dec;20(12):3412-20. doi: 10.1210/me.2006-0312. Epub 2006 Sep 14.
Nuclear receptors are transcriptional regulators that play important roles in embryonic development and organogenesis. To study the potential roles of nuclear receptors in kidney development, we examined the expression patterns of a subset of nuclear receptors in which specific antibodies are available for profiling using immunohistochemistry. As a prototype for our analysis, we investigated the expression patterns of chicken ovalbumin upstream promoter transcription factor (COUP-TF) -I and -II in more details during embryonic development and in the adult by immunohistochemistry. We showed that COUP-TFI is expressed in the stroma and mesenchymal cells at embryonic d 11.5 (E11.5) and expression persists throughout embryonic development. In the adult kidney, only mesangial cells show meaningful COUP-TFI expression. In contrast, COUP-TFII expression is detected as early as E9.5 and high expression is seen in the mesenchymal-derived epithelial cells but not in the ureteric buds through E12.5. At E13.5, COUP-TFII expression becomes regionalized with higher expression in the region that gives rise to the distal tubule. The proximal part of the S-shaped body that will become the glomerulus after endothelial cell migration shows COUP-TFII expression in podocyte precursor cells and epithelial cells of the Bowman's capsule. In the adult mouse kidney, COUP-TFII is detected in distal tubules, podocytes, and the epithelial cells of the Bowman's capsule. In addition to COUP-TFs, we also examined the expression profiles of eight other nuclear receptors (farnesoid X receptor, vitamin D receptor, hepatocyte nuclear factor 4alpha, retinoid X receptor alpha, mineralocorticoid receptor, steroidogenic factor 1, liver receptor homolog-1, and germ cell nuclear factor). Our results suggest that these nuclear receptors are likely to play important physiological roles in the kidney development.
核受体是转录调节因子，在胚胎发育和器官形成中发挥重要作用。为了研究核受体在肾脏发育中的潜在作用，我们检测了一组核受体的表达模式，其中有可用于免疫组织化学分析的特异性抗体。作为我们分析的一个范例，我们通过免疫组织化学更详细地研究了鸡卵清蛋白上游启动子转录因子（COUP-TF）-I和-II在胚胎发育过程中和成年期的表达模式。我们发现，COUP-TFI在胚胎第11.5天（E11.5）的基质和间充质细胞中表达，并且在整个胚胎发育过程中持续表达。在成年肾脏中，只有系膜细胞显示有意义的COUP-TFI表达。相比之下，COUP-TFII的表达最早在E9.5时被检测到，在间充质来源的上皮细胞中高表达，但在E12.5之前的输尿管芽中未检测到。在E13.5时，COUP-TFII的表达变得区域化，在产生远端小管的区域表达较高。在肾小球内皮细胞迁移后将形成肾小球的S形体的近端部分，在足细胞前体细胞和鲍曼囊的上皮细胞中显示COUP-TFII表达。在成年小鼠肾脏中，在远端小管、足细胞和鲍曼囊的上皮细胞中检测到COUP-TFII。除了COUP-TFs，我们还检测了其他八种核受体（法尼醇X受体、维生素D受体、肝细胞核因子4α、视黄酸X受体α、盐皮质激素受体、类固醇生成因子1、肝脏受体同源物-1和生殖细胞核因子）的表达谱。我们的结果表明，这些核受体可能在肾脏发育中发挥重要的生理作用。
