绵羊、山羊和牛R带染色体中LEP和SLC26A2基因的荧光原位杂交定位：牛、羊和山羊4号染色体（BTA4/OAR4/CHI4）与人类7号染色体（HSA7）的比较
FISH-mapping of LEP and SLC26A2 genes in sheep, goat and cattle R-banded chromosomes: comparison between bovine, ovine and caprine chromosome 4 (BTA4/OAR4/CHI4) and human chromosome 7 (HSA7).
作者信息
Perucatti A, Di Meo G P, Vallinoto M, Kierstein G, Schneider M P C, Incarnato D, Caputi Jambrenghi A, Mohammadi G, Vonghia G, Silva A, Brenig B, Iannuzzi L
机构信息
Laboratory of Animal Cytogenetics and Gene Mapping, National Research Council (CNR), ISPAAM, Naples, Italy.
出版信息
Cytogenet Genome Res. 2006;115(1):7-9. doi: 10.1159/000094794.
Sheep (OAR), goat (CHI) and cattle (BTA) R-banded chromosome preparations, obtained from synchronized cell cultures, were used to FISH-map leptin (LEP) and solute carrier family 26 member 2 (SLC26A2) genes on single chromosome bands. LEP maps on OAR4q32 and CHI4q32, being the first assignment of this gene to these two species. SLC26A2 maps on BTA7q24, OAR5q24 and CHI7q24. This gene, too, was assigned for the fist time to both sheep and goat chromosomes, while it was more precisely localized on a single chromosome band in cattle. Improved cytogenetic maps of BTA4/OAR4/CHI4 were constructed and compared with HSA7 revealing five main conserved segments and complex chromosome rearrangements, including a centromere repositioning, differentiating HSA7 and BTA4/OAR4/CHI4.
从同步细胞培养物中获得的绵羊（OAR）、山羊（CHI）和牛（BTA）的R带染色体标本，用于在单个染色体带上对瘦素（LEP）和溶质载体家族26成员2（SLC26A2）基因进行荧光原位杂交定位。LEP定位于OAR4q32和CHI4q32，这是该基因首次在这两个物种上的定位。SLC26A2定位于BTA7q24、OAR5q24和CHI7q24。该基因也是首次定位到绵羊和山羊染色体上，而在牛中它被更精确地定位在单个染色体带上。构建了BTA4/OAR4/CHI4的改进细胞遗传图谱，并与人类7号染色体（HSA7）进行比较，发现了五个主要的保守区段和复杂的染色体重排，包括着丝粒重新定位，这使得HSA7与BTA4/OAR4/CHI4有所不同。
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