利用荧光原位杂交技术对山羊和绵羊中ω干扰素基因与滋养层干扰素基因进行染色体定位
Chromosomal localization of omega and trophoblast interferon genes in goat and sheep by fluorescent in situ hybridization.
作者信息
Iannuzzi L, Di Meo G P, Gallagher D S, Ryan A M, Ferrara L, Womack J E
机构信息
National Research Council, Naples, Italy.
出版信息
J Hered. 1993 Jul-Aug;84(4):301-4. doi: 10.1093/oxfordjournals.jhered.a111343.
Fluorescent in situ hybridization (FISH) of the cattle cDNA probe bTP-509 to goat (Capra hircus L.) and sheep (Ovis aries L.) R-banded metaphase chromosomes localized the omega (IFNW) and trophoblast (IFNT) interferon genes to goat chromosome 8q15 and sheep chromosome 2p15. Caprine chromosome 8 and ovine chromosome 2p are equivalent in banding pattern to cattle chromosome 8. Since FISH has previously localized IFNW and IFNT genes to cattle chromosome 8q15 and river buffalo 3q15, the localization of IFNW and IFNT genes in goat and sheep provides evidence in support of the hypothesis that chromosome-banding homology among these four species is indicative of underlying genetic homology.
用牛cDNA探针bTP - 509对山羊（Capra hircus L.）和绵羊（Ovis aries L.）的R带中期染色体进行荧光原位杂交（FISH），将ω（IFNW）干扰素基因和滋养层（IFNT）干扰素基因定位到山羊8号染色体q15区和绵羊2号染色体p15区。山羊8号染色体和绵羊2号染色体p的带型与牛8号染色体相当。由于之前FISH已将IFNW和IFNT基因定位到牛8号染色体q15区和河水牛3号染色体q15区，IFNW和IFNT基因在山羊和绵羊中的定位为这一假说提供了证据支持，即这四个物种之间的染色体带型同源性表明了潜在的基因同源性。
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