跨越牛MHC III类区域的酵母人工染色体/细菌人工染色体重叠群。

YAC/BAC contig spanning the MHC class III region of cattle.

作者信息

Gelhaus A, Hess M, Förster B, Goldammer T, Schwerin M, Horstmann R D

机构信息

Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

出版信息

Cytogenet Genome Res. 2006;115(1):45-50. doi: 10.1159/000094800.

DOI:10.1159/000094800

PMID:16974083

Abstract

A contig of the class III region of the bovine major histocompatibility complex (MHC) was established from bacterial and yeast artificial chromosomes using PCR and BAC-end sequencing. The marker content of individual clones was determined by gene and BAC-end specific PCR, and the location of genes and BAC-ends was confirmed analyzing somatic hybrid cells. A comparative analysis indicated that the content and order of MHC class III genes is strongly conserved between cattle and other mammalian species. Fluorescence in situ hybridization localized the bovine class III region to BTA23q21-->q22. The results show that the collection of sequenced BAC-ends is a powerful resource for generating high-resolution comparative chromosome maps.

摘要

利用聚合酶链反应（PCR）和细菌人工染色体（BAC）末端测序技术，构建了牛主要组织相容性复合体（MHC）Ⅲ类区域的重叠群。通过基因特异性PCR和BAC末端特异性PCR确定单个克隆的标记物含量，并通过分析体细胞杂种细胞来确认基因和BAC末端的位置。比较分析表明，牛与其他哺乳动物物种之间MHCⅢ类基因的含量和顺序高度保守。荧光原位杂交将牛Ⅲ类区域定位到BTA23q21→q22。结果表明，测序的BAC末端集合是生成高分辨率比较染色体图谱的有力资源。