Walter Lutz, Hurt Peter, Himmelbauer Heinz, Sudbrak Ralf, Günther Eberhard
Abteilung Immungenetik, Universität Göttingen, Heinrich-Düker-Weg 12, 37073 Göttingen, Germany.
Immunogenetics. 2002 Jul;54(4):268-75. doi: 10.1007/s00251-002-0460-x. Epub 2002 Jun 14.
A contig of overlapping bacterial and P1-derived artificial chromosome (BAC, PAC) clones derived from the inbred rat strain BN was constructed that encompasses the class II and the class III regions of the rat MHC (RT1 complex). The genomic structure of the rat, human, and mouse class II and class III regions is highly similar. However, different from human and mouse, a copy of the C4, Cyp21, and Stk19 genes is found that maps to the class II region in the rat. Gene trees constructed from human, rat, and mouse C4, Cyp21, and Stk19 sequences show species-specific clustering of the duplicated genes. The class II/III contig reported here links two previously published PAC contigs of the BN rat that contain the centromeric and the telomeric class I regions, RT1-A and RT1-C/E/M, respectively. Thus, the MHC of the rat is now completely mapped in a single contig of BAC/PAC clones derived from a single RT1 haplotype and encompasses about 3.7 Mb.
构建了一个由重叠的细菌克隆和源自近交大鼠品系BN的P1衍生人工染色体（BAC、PAC）克隆组成的重叠群，该重叠群涵盖大鼠主要组织相容性复合体（RT1复合体）的II类和III类区域。大鼠、人类和小鼠II类和III类区域的基因组结构高度相似。然而，与人类和小鼠不同的是，发现C4、Cyp21和Stk19基因的一个拷贝定位于大鼠的II类区域。根据人类、大鼠和小鼠的C4、Cyp21和Stk19序列构建的基因树显示了重复基因的物种特异性聚类。本文报道的II/III类重叠群连接了BN大鼠先前发表的两个PAC重叠群，它们分别包含着丝粒和端粒I类区域RT1-A和RT1-C/E/M。因此，大鼠的主要组织相容性复合体现在完全定位在源自单个RT1单倍型的BAC/PAC克隆的单个重叠群中，涵盖约3.7 Mb。
