Sykulev Iu K, Eronina T V, Aleshkin V A, Ostreĭko K K
Biokhimiia. 1990 May;55(5):856-64.
In order to demonstrate the participation of galactose-containing carbohydrate epitopes on the surface of model IgG complexes (MIC) during their interaction with high molecular weight ligands, MIC were obtained. The interaction of MIC with Ricinus communis agglutinin (RCA) was studied. The time-dependent changes in the intensity of light scattering in solutions containing MIC of different molecular masses were measured after addition of RCA. It was shown that the efficiency of MIC interaction with RCA depends on the molecular mass of the former. The binding of RCA to MIC is highly specific, it being completely abolished after addition of lactose (1-15 mM). It was found that the final lactose concentration necessary for the complete inhibition of MIC interaction with RCA to take place, depends on the molecular mass of MIC. The data obtained point to the accessibility of IgG oligosaccharide antennae within the composition of MIC for the binding to high molecular weight ligands as well as to the increased density of galactose-containing epitopes on the surface of MIC resulting from the increase in their molecular mass.
为了证明模型IgG复合物（MIC）表面含半乳糖的碳水化合物表位在其与高分子量配体相互作用过程中的参与情况，制备了MIC。研究了MIC与蓖麻凝集素（RCA）的相互作用。在加入RCA后，测量了含有不同分子量MIC的溶液中光散射强度随时间的变化。结果表明，MIC与RCA相互作用的效率取决于前者的分子量。RCA与MIC的结合具有高度特异性，加入乳糖（1 - 15 mM）后结合完全被消除。发现完全抑制MIC与RCA相互作用所需的最终乳糖浓度取决于MIC的分子量。所获得的数据表明，MIC组成中的IgG寡糖天线可用于与高分子量配体结合，并且随着MIC分子量的增加，其表面含半乳糖表位的密度也增加。
