Arima T, Lim D J, Kawaguchi H, Shibata Y, Uemura T
Department of Otolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hear Res. 1990 Jul;46(3):289-92. doi: 10.1016/0378-5955(90)90010-m.
Fine structural features of the 'type A' protofibrils in the guinea pig tectorial membrane were examined using negative staining and deep-etching techniques. Negative-stained samples of fragmented tectorial membrane were composed of several fine filamentous subunits showing the clear banding pattern of the type A protofibrils. Deep-etched replicas of the EGTA (ethylene glycol bis-N,N,N',N'-tetraacetic acid)-treated samples showed fine surface structure consisting of several linear arrays of filamentous elements on the extracellular fibrils, which is interpreted to be type A protofibrils.
利用负染和深蚀刻技术研究了豚鼠盖膜中“A型”原纤维的精细结构特征。破碎盖膜的负染样本由几个细丝状亚基组成，呈现出A型原纤维清晰的条带模式。经乙二醇双（N，N，N'，N'-四乙酸）（EGTA）处理的样本的深蚀刻复制品显示出精细的表面结构，由细胞外原纤维上丝状元件的几个线性阵列组成，这被解释为A型原纤维。
