Schofield D E, Devine W, Yunis E J
Department of Pathology, Children's Hospital of Pittsburgh, Pennsylvania.
J Pediatr Gastroenterol Nutr. 1990 Aug;11(2):221-8. doi: 10.1097/00005176-199008000-00012.
Conflicting reports in the literature regarding the sensitivity and specificity of the acetylcholinesterase (AChE) stain in establishing or excluding the diagnosis of Hirschsprung's disease (HD) prompted this review of 497 rectal biopsies performed on 455 children. Using hematoxylin and eosin (H&E) to stain formalin-fixed, paraffin-embedded tissue sections is our preferred method of identifying ganglion cells. In this series, however, there were eight children with HD, and nine without HD in whom the AChE-stained portion of the sample provided invaluable diagnostic information not obtained from the concomitant, formalin-fixed, H&E-stained portion of the sample. The AChE stain also provided at least suggestive evidence of HD in some of the anal or anorectal biopsy specimens.
文献中关于乙酰胆碱酯酶（AChE）染色在确立或排除先天性巨结肠症（HD）诊断时的敏感性和特异性存在相互矛盾的报道，这促使我们对455名儿童进行的497次直肠活检进行回顾。使用苏木精和伊红（H&E）对福尔马林固定、石蜡包埋的组织切片进行染色是我们识别神经节细胞的首选方法。然而，在这个系列中，有8名患有HD的儿童和9名没有HD的儿童，其样本的AChE染色部分提供了从样本中福尔马林固定、H&E染色的部分未获得的宝贵诊断信息。AChE染色在一些肛门或肛管活检标本中也提供了至少提示HD的证据。
