Golovatskiĭ A S
Arkh Anat Gistol Embriol. 1990 Apr;98(4):61-6.
In the experiment performed on 127 dogs by means of cytospectrofluorometric analysis, using fluorochrome acridine orange in dynamics up to 1 year, changes in the level of chromatin activation and RNA content have been studied in lymphocytes of the germinative centers and the crown of lymphoid nodules, in the paracortical zone and medullary cords of the regional and contralateral popliteal lymph nodes, after subcutaneous injection of antigen (BCG vaccine, 0.2 mg/kg) into the lateral area of the foot of the left pelvic extremity. The immune response is accompanied with a periodical increase in the level of chromatin activation and RNA content in populations of lymphocytes in the regional and contralateral popliteal lymph nodes with maximum in 6 h, 3-7 days, 1-3 months after the antigen injection. The intensity of these processes has an unequal level in lymphoid cells of various structural components; it is higher in lymphocytes of the contralateral lymph node.
在对127只狗进行的实验中，采用细胞分光荧光分析法，在长达1年的动态过程中使用荧光染料吖啶橙，研究了在左下肢足部外侧皮下注射抗原（卡介苗，0.2mg/kg）后，生发中心和淋巴小结冠部、区域及对侧腘淋巴结副皮质区和髓索中淋巴细胞的染色质活化水平和RNA含量的变化。免疫反应伴随着区域及对侧腘淋巴结淋巴细胞群体中染色质活化水平和RNA含量的周期性增加，在抗原注射后6小时、3 - 7天、1 - 3个月达到最大值。这些过程的强度在不同结构成分的淋巴细胞中水平不等；在对侧淋巴结的淋巴细胞中更高。
