Tsvetkova E V, Aleshina G M, Shamova O V, Leonova L E, Lehrer R I, Kokryakov V N
Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, 197376, Russia.
Biochemistry (Mosc). 2006 Aug;71(8):879-83. doi: 10.1134/s0006297906080098.
Three antimicrobial peptides named PHD1-3 (Papio hamadryas defensin) have been isolated from hamadryas baboon blood leukocytes using preparative electrophoresis and reverse-phase HPLC. The primary structures of these peptides have been determined by automated Edman degradation and mass-spectrometry. The results suggest that the peptides belong to the alpha-defensin family. Structural homology analysis reveals that among alpha-defensins from other animal species, PHD3 is the most closely related to RMAD5 (rhesus macaque alpha-defensin) (90% homology) from rhesus macaque leukocytes and also highly similar to human alpha-defensin HD5 (60% homology), which is produced by intestinal Paneth cells. The homology of PHD3 with human neutrophil alpha-defensin HNP1 (human natural peptide) was 30%. The primary structures of PHD1 and PHD2 are most similar to RED1 (rhesus enteral defensin), one of six enteral alpha-defensins of rhesus monkeys. PHD1-3 have been shown to be active against the Gram-positive bacteria Listeria monocytogenes and Staphylococcus aureus, the Gram-negative bacterium Escherichia coli, and the fungus Candida albicans, similarly to the human HNP1 defensin.
利用制备电泳和反相高效液相色谱法，从阿拉伯狒狒血液白细胞中分离出三种抗菌肽，分别命名为PHD1 - 3（阿拉伯狒狒防御素）。这些肽的一级结构已通过自动埃德曼降解法和质谱法确定。结果表明，这些肽属于α-防御素家族。结构同源性分析显示，在来自其他动物物种的α-防御素中，PHD3与恒河猴白细胞中的RMAD5（恒河猴α-防御素）关系最为密切（同源性为90%），也与肠道潘氏细胞产生的人α-防御素HD5高度相似（同源性为60%）。PHD3与人中性粒细胞α-防御素HNP1（人类天然肽）的同源性为30%。PHD1和PHD2的一级结构与恒河猴六种肠道α-防御素之一的RED1（恒河猴肠道防御素）最为相似。与人类HNP1防御素类似，PHD1 - 3已被证明对革兰氏阳性菌单核细胞增生李斯特菌和金黄色葡萄球菌、革兰氏阴性菌大肠杆菌以及真菌白色念珠菌具有活性。
