Hu Xiaoke, Zhao Xueheng, Hwang Huey-Min
Department of Biology, Jackson State University, 1400 Lynch Street, P.O. Box 18540, Jackson, MS 39217, USA.
Chemosphere. 2007 Jan;66(9):1618-26. doi: 10.1016/j.chemosphere.2006.08.004. Epub 2006 Sep 18.
Laccase from Trametes versicolor was immobilized on nanoparticles and kaolinite by physical adsorption or chemical covalence in which the supporters were activated by cross-linked with glutaraldehyde. Thermal and pH stabilities of immobilized laccase on these different supporters were compared. The degradation efficiencies of these immobilized laccases on oxidation of benzo[a]pyrene (BaP) were also compared. The results showed that the immobilized laccases on nanoparticles were more stable in resisting pH and thermal changes. After 48h oxidation, laccase immobilized on kaolinite using the covalent coupling method showed a higher efficiency of oxidation with the BaP residue of 23% in the presence of 1mM HBT and with BaP residue of 37% in 1mM ABTS as the mediator. The results also exhibited a significant inhibition by 1% surfactant Tween 80. According to the HPLC analysis, the oxidation products including 1,6-benzo[a]pyrene quinone, 3,6-benzo[a]pyrene quinone and 6,12-benzo[a]pyrene quinone were identified.
通过物理吸附或化学共价作用将云芝漆酶固定在纳米颗粒和高岭土上，其中载体通过与戊二醛交联进行活化。比较了固定在这些不同载体上的漆酶的热稳定性和pH稳定性。还比较了这些固定化漆酶对苯并[a]芘（BaP）氧化的降解效率。结果表明，固定在纳米颗粒上的漆酶在抵抗pH和热变化方面更稳定。氧化48小时后，使用共价偶联方法固定在高岭土上的漆酶在1mM HBT存在下对BaP的氧化效率更高，BaP残留率为23%，在1mM ABTS作为介质时BaP残留率为37%。结果还显示1%表面活性剂吐温80有显著抑制作用。根据HPLC分析，鉴定出氧化产物包括1,6-苯并[a]芘醌、3,6-苯并[a]芘醌和6,12-苯并[a]芘醌。
