Vukmanovic-Stejic Milica, Reed John R, Lacy Katie E, Rustin Malcolm H A, Akbar Arne N
Department of Immunology and Molecular Pathology, Division of Infection and Immunity, Windeyer Institute of Medical Sciences, University College London, London W1T 4JF, UK.
Immunol Lett. 2006 Nov 15;107(2):93-101. doi: 10.1016/j.imlet.2006.08.002. Epub 2006 Aug 28.
The Mantoux Test (MT) is a classical delayed-type hypersensitivity (DTH) response to the intradermal injection of tuberculin purified protein derivative (PPD). It represents a cutaneous T cell mediated memory recall immune response. The test is typically used to determine immunity to tuberculosis in humans and positive reactions develop in individuals previously exposed to Mycobacterium tuberculosis, and those immunised with the Bacillus of Calmette and Guérin (BCG) vaccine. In view of its relative accessibility human skin represents a convenient tissue for the investigation of human immune responses. Using the MT, we have been able to determine that significant cellular proliferation and clonal expansion occur at the site of antigen deposition in the skin. Furthermore, cells undergoing proliferation in the skin also undergo accelerated differentiation. Taken together with other studies, in humans and in mice, these observations shed new light on the importance of the microenvironment at the site of the immune response for the proliferation and differentiation of memory T cells.
结核菌素试验（MT）是对皮内注射结核菌素纯蛋白衍生物（PPD）产生的经典迟发型超敏反应（DTH）。它代表一种皮肤T细胞介导的记忆性回忆免疫反应。该试验通常用于确定人类对结核病的免疫力，先前接触过结核分枝杆菌的个体以及接种过卡介苗（BCG）的个体中会出现阳性反应。鉴于人类皮肤相对易于获取，它是研究人类免疫反应的便利组织。通过结核菌素试验，我们已经能够确定在皮肤抗原沉积部位会发生显著的细胞增殖和克隆扩增。此外，在皮肤中增殖的细胞也会加速分化。与人类和小鼠的其他研究一起，这些观察结果为免疫反应部位的微环境对记忆T细胞增殖和分化的重要性提供了新的见解。
