Webb D S, Shimizu Y, Van Seventer G A, Shaw S, Gerrard T L
Food and Drug Administration, Division of Cytokine Biology, Bethesda, MD 20892.
Science. 1990 Sep 14;249(4974):1295-7. doi: 10.1126/science.1697984.
The monocyte-derived cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), are central regulators of the immune response, but the physiologic stimuli for their release remain largely undefined. Engagement of three monocyte glycoproteins, LFA-3, CD44, and CD45, by specific monoclonal antibodies immobilized on plastic induced TNF-alpha and IL-1 beta release. In addition, TNF-alpha was released when monocyte LFA-3 bound immobilized, purified CD2, which is its physiologic receptor. Thus, a receptor-ligand interaction that mediates cell-cell adhesion can transmit the necessary signals for the release of monokines.
单核细胞衍生的细胞因子，肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β），是免疫反应的核心调节因子，但其释放的生理刺激因素在很大程度上仍不明确。固定在塑料上的特异性单克隆抗体与三种单核细胞糖蛋白，即淋巴细胞功能相关抗原-3（LFA-3）、CD44和CD45结合，可诱导TNF-α和IL-1β的释放。此外，当单核细胞LFA-3与固定化的纯化CD2（其生理受体）结合时，TNF-α会释放。因此，介导细胞间粘附的受体-配体相互作用可以传递释放单核因子所需的信号。
