Dutta S K
Avian Dis. 1975 Jul-Sep;19(3):429-36.
Chicken tracheas infected in vitro in organ culture and in vivo with avian infectious bronchitis virus (IBV) were studied by scanning electron microscopy. In in vitro infection in organ culture the columnar epithelial cells lining the tracheal ring lost their cilia and rounded up in 48 hr. There were numerous elevations and holes on the infected cell surface. Subsequently the cells sloughed off. In in vivo infection the tracheal epithelial cells lost their cilia in 4 days. These cells without cilia appeared bulging, and the surfaces were covered with microvillus-like structures. Subsequently a pseudomembrane was present in most areas. In 10 days postinfection the cilia started regenerating, and in 12 days postinfection there was complete regeneration of cells and cilia, giving a normal appearance to the trachea.
采用扫描电子显微镜对在器官培养中体外感染以及在体内感染禽传染性支气管炎病毒（IBV）的鸡气管进行了研究。在器官培养的体外感染中，气管环内衬的柱状上皮细胞在48小时内失去纤毛并变圆。受感染细胞表面有许多隆起和孔洞。随后细胞脱落。在体内感染中，气管上皮细胞在4天内失去纤毛。这些没有纤毛的细胞出现肿胀，表面覆盖着微绒毛样结构。随后大多数区域出现假膜。感染后10天纤毛开始再生，感染后12天细胞和纤毛完全再生，气管外观恢复正常。
