Bing P, Maode L, Li F, Sheng H
Surgery Department, West China Hospital, SiChuan University, Guoxuexiang 37, Chengdu 610041, P.R. China.
Transplant Proc. 2006 Sep;38(7):2176-9. doi: 10.1016/j.transproceed.2006.07.015.
We sought to detect expression of transforming growth factor-beta 1 (TGF-beta1) as well as its receptors type I (TRI) and type II (TRII) in rat kidneys during chronic cyclosporine (CsA)-induced nephropathy.
Twenty four rats were randomly divided into three experimental groups: group 1; NSD (control, n = 8) were administered a normal sodium diet, group 2; LSD (n = 8) were administered a low sodium diet, group 3; CsA (n = 8) were sodium-depleted rats administered Neoral by gastric gavage in a model of chronic CsA-induced nephropathy. TGF-beta1, TRI, and TRII proteins, as well as TRI and TRII mRNAs were measured in the CsA-treated rat kidneys by immunohistochemistry and in situ hybridization, respectively. Semiquantitative results were shown by image analysis.
The expression of TGF-beta1, TRI, TRII, TRI mRNA, and TRII mRNA were all increased in CsA-treated rat kidneys, compared with NSD or LSD (P < .05).
Our study showed that the ligand of TGF-beta1 and its receptors TRI, TRII were all up-regulated. It may be important to inhibit the expression of TGF-beta1 or its receptors in patients who suffer from chronic CsA-induced nephropathy.
我们试图检测在慢性环孢素（CsA）诱导的大鼠肾病模型中，大鼠肾脏中转化生长因子-β1（TGF-β1）及其Ⅰ型受体（TRⅠ）和Ⅱ型受体（TRⅡ）的表达情况。
将24只大鼠随机分为三个实验组：第1组；NSD（对照组，n = 8）给予正常钠饮食，第2组；LSD（n = 8）给予低钠饮食，第3组；CsA（n = 8）为在慢性CsA诱导的肾病模型中通过胃管饲法给予Neoral的低钠大鼠。分别通过免疫组织化学和原位杂交检测CsA处理的大鼠肾脏中TGF-β1、TRⅠ和TRⅡ蛋白以及TRⅠ和TRⅡ mRNA。通过图像分析显示半定量结果。
与NSD或LSD相比，CsA处理的大鼠肾脏中TGF-β1、TRⅠ、TRⅡ、TRⅠ mRNA和TRⅡ mRNA的表达均增加（P < .05）。
我们的研究表明，TGF-β1的配体及其受体TRⅠ、TRⅡ均上调。对于患有慢性CsA诱导的肾病的患者，抑制TGF-β1或其受体的表达可能很重要。
