基于包膜序列对中国猪中表达的猪内源性逆转录病毒进行系统发育分析。

Phylogenetic analysis of porcine endogenous retroviruses expressed in Chinese pigs based on envelope sequences.

作者信息

Li Z, Ping Y, Shengfu L, Youping L, Jingqiu C, Hong B

机构信息

Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, P.R. China.

出版信息

Transplant Proc. 2006 Sep;38(7):2252-7. doi: 10.1016/j.transproceed.2006.06.097.

DOI:10.1016/j.transproceed.2006.06.097

PMID:16980057

Abstract

The promise of successful clinical xenotransplantation is now offset by the potential risk of transmission of porcine endogenous retrovirus (PERV). PERV consists of three subtypes according to the varieties of env sequences. We analyzed PERV subtypes in two species of Chinese pigs (Banna minipig inbred, BMI, and Wu-Zhi-Shan pig, WZSP). Positive A and B were detected while positive C was absent in the analyzed Chinese pigs. The polymerase chain reaction products were then cloned into a pGEM-T vector system and sequenced. Phylogenetic trees were constructed from the translated amino acids of PERVs and other type C and type D retrovirus, as well as the lentivirus in the GeneBank. The results suggested that PERV-A and PERV-B that exist in Chinese pig genomes share similarities with other PERV from the GeneBank and some type C retroviruses, including lymphotropic, leukemic and endogenous retroviruses.

摘要

成功进行临床异种移植的前景目前因猪内源性逆转录病毒（PERV）传播的潜在风险而受到影响。根据env序列的种类，PERV由三种亚型组成。我们分析了两种中国猪（版纳微型猪近交系，BMI，和五指山猪，WZSP）中的PERV亚型。在所分析的中国猪中检测到了A和B亚型阳性，而未检测到C亚型阳性。然后将聚合酶链反应产物克隆到pGEM-T载体系统中并进行测序。根据PERV以及基因库中其他C型和D型逆转录病毒、慢病毒的翻译氨基酸构建了系统发育树。结果表明，中国猪基因组中存在的PERV-A和PERV-B与基因库中的其他PERV以及一些C型逆转录病毒具有相似性，包括嗜淋巴细胞性、白血病性和内源性逆转录病毒。