Zhang Li, Bu Hong, Li Shengfu, Yu Ping, Li Youping, Cheng Jingqiu
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):197-9.
To analyze the subtype of porcine endogenous retrovirus (PERV) in two species of Chinese pigs-Wu zhishan pig and Banna minipig inbred; the total number of pigs being eighty six.
Three primers which are specific for the three PERV subtypes (subtypes A, B and C) were used to amplify the genome DNA extracted from the pigs.
In all tested pigs' genome, subtype C was not found; in 77.9% pigs, double positive envAB were found; in 22.1% pigs, only A or B was found.
In the genome of peripheral leukocytes from Wuzhishan pig and Banna minipig inbred, only subtypes A and B exist, and envAB predominate.
分析两种中国猪——五指山猪和版纳微型猪近交系中猪内源性逆转录病毒（PERV）的亚型；猪的总数为86头。
使用针对三种PERV亚型（A、B和C亚型）的三种引物，对从猪中提取的基因组DNA进行扩增。
在所有检测猪的基因组中，未发现C亚型；77.9%的猪中发现envAB双阳性；22.1%的猪中仅发现A或B亚型。
在五指山猪和版纳微型猪近交系外周血白细胞基因组中，仅存在A和B亚型，且envAB占主导。
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