Ebert E C, Roberts A I, Devereux D, Nagase H
Department of Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick 08903-0019.
Cancer Res. 1990 Oct 1;50(19):6158-61.
A soluble substance (HT29 factor) produced by HT29 colon cancer cells markedly suppresses mitogen-induced T-cell proliferation and interleukin-2 production. In this study the range of T-cell functions susceptible to the inhibitory effects of the HT29 factor was evaluated. Serum-free conditioned medium was collected from confluent cultures of HT29 cancer cells, concentrated, and subjected to gel filtration chromatography and anion exchange chromatography, resulting in 24.4-fold purification of the HT29 factor with 31% yield. This factor abolished the development of lymphokine-activated killer cells when present during activation of peripheral blood lymphocytes by interleukin-2 but did not affect the lysis of target cells by normal effectors when added in the lysis stage only. The HT29 factor did not affect the generation of concanavalin A-induced suppressor lymphocytes, even though it markedly inhibited synthesis of DNA, RNA, and protein as well as expression of the CD25 (Tac) antigen during mitogen activation of T-cells. The HT29 factor itself did not induce suppressor cells. These results indicate that the immunosuppressive action of the HT29 factor is selective.
HT29结肠癌细胞产生的一种可溶性物质（HT29因子）能显著抑制有丝分裂原诱导的T细胞增殖和白细胞介素-2的产生。在本研究中，评估了易受HT29因子抑制作用影响的T细胞功能范围。从HT29癌细胞的汇合培养物中收集无血清条件培养基，浓缩后进行凝胶过滤色谱和阴离子交换色谱，得到了纯化24.4倍、产率为31%的HT29因子。当该因子在白细胞介素-2激活外周血淋巴细胞的过程中存在时，它会抑制淋巴因子激活的杀伤细胞的发育，但仅在裂解阶段加入时，它并不影响正常效应细胞对靶细胞的裂解。HT29因子并不影响伴刀豆球蛋白A诱导的抑制性淋巴细胞的产生，尽管它在T细胞有丝分裂原激活过程中能显著抑制DNA、RNA和蛋白质的合成以及CD25（Tac）抗原的表达。HT29因子本身不会诱导抑制性细胞。这些结果表明，HT29因子的免疫抑制作用具有选择性。
