Baba S, Abe Y, Ono C, Hosobuchi M
Process Development Laboratories, Sankyo Co., Ltd., Fukushima, Japan.
Biochim Biophys Acta. 2006 Aug-Sep;1759(8-9):410-6. doi: 10.1016/j.bbaexp.2006.08.001.
The role of two genes, mlcR and ariB, was investigated by gene disruption experiments. The mlcR gene in the ML-236B biosynthetic gene cluster of Penicillium citrinum encodes a putative 50.2-kDa protein with a Zn (II) 2Cys6 DNA-binding domain, and has similarity to most of the GAL4-type regulatory proteins. The mlcR disruptant did not produce ML-236B or its intermediates, suggesting that mlcR is involved in ML-236B biosynthesis. Transcriptional analysis of the mlcR disruptant by Northern hybridization and RT-PCR indicated that MlcR activates the transcription of mlcA, B, C,D, F, G and H in a pathway-specific manner. On the other hand, MlcR did not affect the transcription of mlcE and the genes outside the ML-236B cluster. The ariB gene, next to mlcR, encodes another GAL4-type protein. Transcriptional analysis of the ariB disruptant indicated that it is a transcriptional activator of the genes outside the ML-236B cluster, and is not related to ML-236B biosynthesis.
通过基因敲除实验研究了两个基因mlcR和ariB的作用。桔青霉ML-236B生物合成基因簇中的mlcR基因编码一种推定的50.2 kDa蛋白，具有Zn (II) 2Cys6 DNA结合结构域，与大多数GAL4型调节蛋白相似。mlcR敲除突变体不产生ML-236B或其中间体，表明mlcR参与ML-236B的生物合成。通过Northern杂交和RT-PCR对mlcR敲除突变体进行转录分析表明，MlcR以途径特异性方式激活mlcA、B、C、D、F、G和H的转录。另一方面，MlcR不影响mlcE和ML-236B簇外基因的转录。紧挨着mlcR的ariB基因编码另一种GAL4型蛋白。对ariB敲除突变体的转录分析表明，它是ML-236B簇外基因的转录激活因子，与ML-236B的生物合成无关。
