Hansbury M J, McIlroy P J, Goldenthal M J
Department of Biology, Rutgers University, Camden, NJ 08102.
Biochem Biophys Res Commun. 1990 Sep 14;171(2):525-30. doi: 10.1016/0006-291x(90)91177-t.
Molecular analysis of the induction of the lutropin/choriogonadotropin receptor during the process of luteinization of the rat ovary was performed. The appearance of receptor binding activity and an immunological analysis of the receptor using Triton solubilized membrane proteins show little receptor present in luteal tissue through day 3 subsequent to hCG treatment, with some in day 4, and a marked increase by day 5. A similar pattern was found in the analysis of RNA hybridizing to several probes derived from the published cDNA sequence suggesting that receptor induction occurs primarily at the level of transcription.
对大鼠卵巢黄体化过程中促黄体生成素/绒毛膜促性腺激素受体诱导进行了分子分析。利用Triton溶解的膜蛋白对受体结合活性进行检测以及对受体进行免疫分析，结果显示，在人绒毛膜促性腺激素（hCG）处理后的第3天，黄体组织中几乎没有受体，第4天有一些受体，到第5天受体显著增加。在与从已发表的cDNA序列衍生的几种探针杂交的RNA分析中发现了类似的模式，这表明受体诱导主要发生在转录水平。
