Goossens M
Service de Biochimie, INSERM U91, Hôpital Henri-Mondor, Créteil, France.
Reprod Nutr Dev. 1990;Suppl 1:117s-124s.
The polymerase chain reaction (PCR) is a method for selective amplification of DNA or RNA segments of up to 2 kilobase pairs (kb) or more in length. Repeated cycles of enzymatic primer extension in opposite directions are performed using synthetic oligonucleotides flanking the sequences of interest and the heat-stable DNA polymerase from the archaebacterium Thermus aquaticus (Taq polymerase). The reaction is simple, fast and extremely sensitive, and the DNA or RNA content of a single cell is sufficient to detect a specific sequence. This method has proved useful in simplifying complex analytical protocols in basic and applied molecular biology.
聚合酶链反应（PCR）是一种用于选择性扩增长度达2千碱基对（kb）或更长的DNA或RNA片段的方法。使用位于目标序列两侧的合成寡核苷酸和来自嗜热水生栖热菌的热稳定DNA聚合酶（Taq聚合酶），在相反方向上进行酶促引物延伸的重复循环。该反应简单、快速且极其灵敏，单个细胞的DNA或RNA含量就足以检测特定序列。这种方法已被证明在简化基础和应用分子生物学中的复杂分析方案方面很有用。
