Jin Zhe-Long, Tada Atsuko, Sugimoto Naoki, Sato Kyoko, Masuda Aino, Yamagata Kazuo, Yamazaki Takeshi, Tanamoto Kenichi
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Shokuhin Eiseigaku Zasshi. 2006 Aug;47(4):167-72. doi: 10.3358/shokueishi.47.167.
Urushi wax is a natural gum base used as a food additive. In order to evaluate the quality of urushi wax as a food additive and to obtain information useful for setting official standards, we investigated the constituents and their concentrations in urushi wax, using the same sample as scheduled for toxicity testing. After methanolysis of urushi wax, the composition of fatty acids was analyzed by GC/MS. The results indicated that the main fatty acids were palmitic acid, oleic acid and stearic acid. LC/MS analysis of urushi wax provided molecular-related ions of the main constituents. The main constituents were identified as triglycerides, namely glyceryl tripalmitate (30.7%), glyceryl dipalmitate monooleate (21.2%), glyceryl dioleate monopalmitate (2.1%), glyceryl monooleate monopalmitate monostearate (2.6%), glyceryl dipalmitate monostearate (5.6%), glyceryl distearate monopalmitate (1.4%). Glyceryl dipalmitate monooleate isomers differing in the binding sites of each constituent fatty acid could be separately determined by LC/MS/MS.
漆蜡是一种用作食品添加剂的天然树胶基料。为了评估漆蜡作为食品添加剂的质量，并获取有助于制定官方标准的信息，我们使用与毒性测试计划相同的样品，对漆蜡的成分及其含量进行了调查。漆蜡经甲醇解后，通过气相色谱/质谱联用仪（GC/MS）分析脂肪酸的组成。结果表明，主要脂肪酸为棕榈酸、油酸和硬脂酸。对漆蜡进行液相色谱/质谱联用仪（LC/MS）分析，得到了主要成分的分子相关离子。主要成分被鉴定为甘油三酯，即三棕榈酸甘油酯（30.7%）、二棕榈酸单油酸甘油酯（21.2%）、二油酸单棕榈酸甘油酯（2.1%）、单油酸单棕榈酸单硬脂酸甘油酯（2.6%）、二棕榈酸单硬脂酸甘油酯（5.6%）、二硬脂酸单棕榈酸甘油酯（1.4%）。通过液相色谱/串联质谱联用仪（LC/MS/MS）可以分别测定各组成脂肪酸结合位点不同的二棕榈酸单油酸甘油酯异构体。
