Shimoi Toshiko, Ushiyama Hirofumi, Kan Kimiko, Saito Kazuo, Kamata Kunihiro
Tokyo Metropolitan Institute of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Shokuhin Eiseigaku Zasshi. 2006 Aug;47(4):189-95. doi: 10.3358/shokueishi.47.189.
Allergic substances (eggs, milk) in commercial foods were measured by means of the notified ELISA methods using FASTKIT ELISA kit (N kit) and "Tokuteigenzairyo sokutei kit" (M kit). Some samples were also analyzed with the notified western blot method using "Tokuteigenzairyo western blot kit". In the methods for detection of eggs, proteins of fish egg and chicken muscle were examined using the ELISA and Western blotting methods to check for cross-reaction. Sujiko was false positive with the M kit. Difficulties were also encountered in the notified ELISA and Western blotting methods with raw chicken. In foods labeled as containing eggs, it was difficult to detected in the heart-processed food using both notified methods. In the method for detection of milk, foods containing thickening polysaccharides, showed cross-reaction with the N kit. However, this cross-reaction was eliminated when an improved N kit was used. Foods labeled as containing milk did not present any difficulty.
使用FASTKIT ELISA试剂盒（N试剂盒）和“特定原材料测定试剂盒”（M试剂盒），通过已通报的ELISA方法对市售食品中的过敏原物质（鸡蛋、牛奶）进行了测定。部分样品还使用“特定原材料western blot试剂盒”通过已通报的western blot方法进行了分析。在鸡蛋检测方法中，采用ELISA和western blot方法检测鱼卵和鸡肉的蛋白质，以检查交叉反应情况。寿喜锅（sujiko）用M试剂盒检测呈假阳性。生鸡肉采用已通报的ELISA和western blot方法检测时也遇到了困难。在标记含有鸡蛋的食品中，采用两种已通报方法在深加工食品中均难以检测到。在牛奶检测方法中，含有增稠多糖的食品与N试剂盒发生交叉反应。不过，使用改进后的N试剂盒时，这种交叉反应得以消除。标记含有牛奶的食品检测未出现任何困难。
