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一种定位于担子菌灰盖鬼伞幼担子果菌盖中的泛素免疫反应蛋白的纯化与特性分析

Purification and characterization of an ubiquitin-immuno-reactive protein localized in the cap of young basidiocarp in the basidiomycete Coprinus cinereus.

作者信息

Kanda T, Inoue M, Akiyama M

机构信息

Department of Biology, Faculty of Science, Okayama University, Japan.

出版信息

Biochimie. 1990 May;72(5):355-9. doi: 10.1016/0300-9084(90)90032-c.

Abstract

The ubiquitin-immuno-reactive protein with a molecular weight of 27,800 daltons, which is mainly present in the cap of young basidiocarp, was purified from the basidiomycete Coprinus cinereus. The molecular weight of the native protein was approximately 55,000 as determined by gel filtration chromatography. The isoelectric point of the protein was 4.4. The amino-terminal sequence of the protein was also determined.

摘要

从灰盖鬼伞中纯化出一种分子量为27,800道尔顿的泛素免疫反应性蛋白,该蛋白主要存在于幼嫩担子果的菌盖中。通过凝胶过滤色谱法测定,天然蛋白的分子量约为55,000。该蛋白的等电点为4.4。还测定了该蛋白的氨基末端序列。

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