Kanda T, Inoue M, Akiyama M
Department of Biology, Faculty of Science, Okayama University, Japan.
Biochimie. 1990 May;72(5):355-9. doi: 10.1016/0300-9084(90)90032-c.
The ubiquitin-immuno-reactive protein with a molecular weight of 27,800 daltons, which is mainly present in the cap of young basidiocarp, was purified from the basidiomycete Coprinus cinereus. The molecular weight of the native protein was approximately 55,000 as determined by gel filtration chromatography. The isoelectric point of the protein was 4.4. The amino-terminal sequence of the protein was also determined.
从灰盖鬼伞中纯化出一种分子量为27,800道尔顿的泛素免疫反应性蛋白,该蛋白主要存在于幼嫩担子果的菌盖中。通过凝胶过滤色谱法测定,天然蛋白的分子量约为55,000。该蛋白的等电点为4.4。还测定了该蛋白的氨基末端序列。