Moulavi Fariba, Hosseini Sayyed Mortaza, Ashtiani Saeid Kazemi, Shahverdi Abdolhossein, Nasr-Esfahani Mohammad Hossein
Royan Institute, Tehran, Iran.
Reprod Biomed Online. 2006 Sep;13(3):404-11. doi: 10.1016/s1472-6483(10)61446-0.
This study was carried out to evaluate the effect of Vero cell co-culture on developmental competence of immature oocytes. Bovine cumulus-oocyte complexes (COC) were matured in presence or absence of Vero cells. Matured oocytes were inseminated and cultured for up to 9 days. Cleavage percentages were recorded on day 2 after insemination and embryos were evaluated on a daily basis. Expanding/expanded and hatching/hatched blastocysts were used for cell number assay. Results indicated a significantly greater cleavage percentage in oocytes matured in presence of Vero cells than control (86% versus 76%, P < or = 0.05). The percentages of advanced embryos appear to be greater on a daily basis in COC matured in presence of Vero cells compared with control. However, these differences were not significant. Blastocysts derived from COC matured in the presence of Vero cells had a significantly higher (P < or = 0.05) number of inner cell mass, trophectoderm and total cell number in expanding/expanded (65.25, 224.5 and 289.7 respectively) and hatching/hatched (67.75, 289.75 and 357.5) embryos in comparison to the control (42, 203.5, 245.5 and 51.3, 265, 316.3 respectively). Results confirm that co-culture of bovine COC during in-vitro maturation, enhances their ability for cleavage and for producing blastocysts with higher quality.
本研究旨在评估Vero细胞共培养对未成熟卵母细胞发育能力的影响。牛卵丘-卵母细胞复合体（COC）在有或无Vero细胞存在的情况下进行成熟培养。成熟的卵母细胞进行受精并培养长达9天。在受精后第2天记录分裂率，并每天对胚胎进行评估。将扩张/已扩张和孵化/已孵化的囊胚用于细胞数量测定。结果表明，在Vero细胞存在下成熟的卵母细胞的分裂率显著高于对照组（86%对76%，P≤0.05）。与对照组相比，在Vero细胞存在下成熟的COC中，每天发育至更高级阶段胚胎的比例似乎更高。然而，这些差异并不显著。与对照组（分别为42、203.5、245.5和51.3、265、316.3）相比，在Vero细胞存在下成熟的COC所衍生的囊胚，在扩张/已扩张（分别为65.25、224.5和289.7）和孵化/已孵化（分别为67.75、289.75和357.5）胚胎中的内细胞团、滋养外胚层和总细胞数量显著更高（P≤0.05）。结果证实，牛COC在体外成熟过程中共培养，可提高其分裂能力以及产生更高质量囊胚的能力。
