Bressler R B, Friedman M M, Kirshenbaum A S, Irani A M, Schwartz L B, Metcalfe D D
Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.
Int Arch Allergy Appl Immunol. 1990;91(4):403-10. doi: 10.1159/000235150.
Human bone marrow cells grown in liquid culture in the presence of conditioned medium from concanavalin A stimulated peripheral blood mononuclear cells, from mixed lymphocyte reactions, or from an osteogenic sarcoma tumor line gave rise to basophils which were maximal in number at 1-3 weeks. Basophils had a multilobed nucleus, contained large cytoplasmic granules of variable size which stained metachromatically with acid, but not neutral toluidine blue, were negative for chloroacetate esterase, and did not contain human mast cell tryptase. In long-term cultures, mast cells were detected after 5-6 weeks. Mast cells had cytoplasmic granules that stained with acid and neutral toluidine blue, were positive for chloroacetate esterase, and contained human mast cell tryptase. It, therefore, appears that in liquid cultures even in the absence of feeder layers, human bone marrow cell cultures can give rise initially to basophils, and then to mast cells.
在伴刀豆球蛋白A刺激的外周血单个核细胞、混合淋巴细胞反应或骨肉瘤肿瘤系的条件培养基存在下，在液体培养中生长的人骨髓细胞产生了嗜碱性粒细胞，其数量在1至3周时达到最大值。嗜碱性粒细胞有分叶核，含有大小不一的大细胞质颗粒，这些颗粒用酸性但非中性甲苯胺蓝进行异染性染色，氯乙酸酯酶呈阴性，且不含有人类肥大细胞类胰蛋白酶。在长期培养中，5至6周后检测到肥大细胞。肥大细胞有细胞质颗粒，用酸性和中性甲苯胺蓝染色，氯乙酸酯酶呈阳性，且含有人类肥大细胞类胰蛋白酶。因此，似乎在液体培养中，即使没有饲养层，人骨髓细胞培养最初也能产生嗜碱性粒细胞，然后产生肥大细胞。
