Tanaka Y, Yasumoto M, Nyunoya H, Ogura T, Kikuchi M, Shimotohno K, Shiraki H, Kuroda N, Shida H, Tozawa H
Department of Immunology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
Int J Cancer. 1990 Oct 15;46(4):675-81. doi: 10.1002/ijc.2910460421.
In order to study the antigenicity of envelope 46 kDa glycoprotein (gp46) of human T-cell leukemia virus type-I (HTLV-1), we have generated monoclonal anti-gp46 antibodies (MAbs), REY-7, REY-11, REY-16, REY-30, MET-2 and MET-3 from rats and mice. Immunoblot and immunofluorescence assays showed that these MAbs recognize gp46 and its related antigens, and specifically stained HTLV-I-bearing cells. All MAbs reacted with a recombinant gp46 antigen, N147, expressing the 147 amino acids in the C-terminal half of gp46. By using various synthetic peptides corresponding to the gp46 sequence, epitopes recognized by REY-7 and MET-3, REY-11 and REY-16, and REY-30 were mapped to regions corresponding to the amino acids 175-199, 253-282 and 288-312, respectively. MET-2 did not react with any of the peptides used. These results indicate that the present MABs are directed against at least 4 distinct epitopes expressed on the C-terminal half of gp46. The binding of these MAbs to gp46 was specifically inhibited by sera from HTLV-I-infected individuals, but none of these MAbs inhibited the cell fusion activity of HTLV-I.
为研究I型人类T细胞白血病病毒（HTLV-1）包膜46 kDa糖蛋白（gp46）的抗原性，我们从大鼠和小鼠体内制备了单克隆抗gp46抗体（MAb），即REY-7、REY-11、REY-16、REY-30、MET-2和MET-3。免疫印迹和免疫荧光分析表明，这些单克隆抗体可识别gp46及其相关抗原，并特异性地对携带HTLV-I的细胞进行染色。所有单克隆抗体均与重组gp46抗原N147发生反应，N147表达gp46 C端一半的147个氨基酸。通过使用与gp46序列对应的各种合成肽，REY-7和MET-3、REY-11和REY-16以及REY-30识别的表位分别被定位到对应于氨基酸175 - 199、253 - 282和288 - 312的区域。MET-2与所使用的任何肽均无反应。这些结果表明，目前的单克隆抗体针对gp46 C端一半表达的至少4个不同表位。这些单克隆抗体与gp46的结合被HTLV-I感染个体的血清特异性抑制，但这些单克隆抗体均未抑制HTLV-I的细胞融合活性。
