Raucci Franca, Di Fiore Maria M, Pinelli Claudia, D'Aniello Biagio, Luongo Luciano, Polese Gianluca, Rastogi Rakesh K
Department of Life Sciences, Second University of Naples, 81100 Caserta, Italy.
J Chem Neuroanat. 2006 Dec;32(2-4):127-42. doi: 10.1016/j.jchemneu.2006.08.001. Epub 2006 Sep 20.
By means proliferating cell nuclear antigen (PCNA) immunohistochemistry, we have provided a detailed neuroanatomical mapping of proliferative activity during development and adulthood in the frog (Rana esculenta) brain. Western blot analysis confirmed the presence of this protein in brain extracts from adults and tadpoles. Proliferative activity was observed in the ventricular and subventricular zones throughout the brain. The present study provides details as to which of the morphologically distinguishable brain region(s) has a long-lasting proliferative activity and in which region this activity undergoes a progressive decrease during development. In the subventricular zones of the third ventricle, PCNA-labeled cells were particularly abundant in the magnocellular preoptic nucleus and the ventromedial thalamic nucleus. It was observed that proliferation zones are present practically in all major subdivisions of the forebrain, midbrain and hindbrain, including the cerebellum in which PCNA-labeled cells were located in the outer granular layer and the inner molecular layer. The habenulae, epiphysis and isthmic nuclei never showed the presence of PCNA-immunoreactive nuclei. The widespread proliferative activity implies that the frog brain has a great potential for neurogenesis/gliogenesis not only during larval development but also in the adulthood.
通过增殖细胞核抗原（PCNA）免疫组织化学方法，我们对食用蛙（Rana esculenta）大脑发育和成年期的增殖活性进行了详细的神经解剖学定位。蛋白质印迹分析证实该蛋白存在于成年蛙和蝌蚪的脑提取物中。在整个大脑的脑室和脑室下区均观察到增殖活性。本研究详细说明了哪些形态上可区分的脑区具有持久的增殖活性，以及在发育过程中哪些区域的这种活性会逐渐降低。在第三脑室的脑室下区，PCNA标记的细胞在大细胞视前核和腹内侧丘脑核中特别丰富。据观察，增殖区实际上存在于前脑、中脑和后脑的所有主要亚区，包括小脑，其中PCNA标记的细胞位于外颗粒层和内分子层。缰核、松果体和峡核从未显示出PCNA免疫反应性细胞核的存在。广泛的增殖活性表明，蛙脑不仅在幼体发育期间，而且在成年期都具有巨大的神经发生/胶质发生潜力。
