Abbasi S, Esfandyarpour M, Taher M A, Daneshfar A
Department of Chemistry, Ilam University, Ilam, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Jul;67(3-4):578-81. doi: 10.1016/j.saa.2006.06.046. Epub 2006 Aug 18.
A new simple and rapid catalytic kinetic method for the determination of trace amount of formaldehyde is described. The method is based on the catalytic effect of formaldehyde on the oxidation of Janus green by bromate in the present of sulfuric acid. The reaction monitored spectrophotometrically by measuring the decrease in absorbance of the reaction mixture at 618 nm. The fixed-time method was used for the first 150 s. For initiation of the reaction, under the optimum conditions, in the concentration range of 0.003-2.5 microg ml(-1) formaldehyde can be determined with a limit of detection 0.0015 microg ml(-1). The relative standard deviation of five replicate measurements is 2.3% for 1.0 microg ml(-1) of formaldehyde. The method was used for the determination of formaldehyde in real samples with satisfactory results.
描述了一种测定痕量甲醛的新型简单快速催化动力学方法。该方法基于在硫酸存在下，甲醛对溴酸盐氧化天青的催化作用。通过测量反应混合物在618nm处吸光度的降低，用分光光度法监测反应。在前150s使用固定时间法。在最佳条件下引发反应，在0.003 - 2.5μg ml(-1)浓度范围内可测定甲醛，检测限为0.0015μg ml(-1)。对于1.0μg ml(-1)的甲醛，五次重复测量的相对标准偏差为2.3%。该方法用于实际样品中甲醛的测定，结果令人满意。
