Lee Kyoung-Young, Kim Su-Jong, Cha Youn-Soo, So Ju-Ryun, Park Joon-Suk, Kang Kyung-Sun, Chon Tae-Won
Department of Physical Education, College of Education, Seoul National University, Seoul, Korea.
Obesity (Silver Spring). 2006 Aug;14(8):1294-302. doi: 10.1038/oby.2006.147.
To understand the molecular mechanisms involved in the effect of exercise training, we examined hepatic transcriptional profiles using cDNA microarrays in exercise-trained and untrained mice with diet-induced obesity. C57BL/6J male mice (n = 10/group) were fed with a normal diet, high-fat diet (HFD), or HFD with exercise training for 12 weeks. The expression level of approximately 10,000 transcripts in liver tissues from each group was assessed using cDNA microarray analysis. Exercise training improved lipid profiles and hepatic steatosis and decreased body fat mass induced by the HFD. Seventy-three genes were differentially expressed in the HFD- and/or HFD with exercise training-treated groups, compared with the normal diet- and HFD-fed groups, respectively. Interestingly, the expression profiles involved in metabolism, such as elongation of very long chain fatty acids-like 2, lipin, and malic enzyme, were changed by exercise training. In addition, expression of genes altered by exercise training related to defense and stress response, including metallothionein 1 and 2 and heat shock protein, showed interesting findings. Our study showed beneficial effects of exercise training in preventing the development of obesity and metabolic disorders in mice with diet-induced obesity.
为了解运动训练效果所涉及的分子机制，我们使用cDNA微阵列检测了饮食诱导肥胖的运动训练小鼠和未训练小鼠的肝脏转录谱。将C57BL/6J雄性小鼠（每组n = 10）喂食正常饮食、高脂饮食（HFD）或高脂饮食并进行运动训练12周。使用cDNA微阵列分析评估每组肝脏组织中约10,000个转录本的表达水平。运动训练改善了脂质谱和肝脂肪变性，并减少了高脂饮食诱导的体脂量。与正常饮食组和高脂饮食组相比，分别有73个基因在高脂饮食组和/或高脂饮食加运动训练组中差异表达。有趣的是，运动训练改变了与代谢相关的表达谱，如超长链脂肪酸延伸样2、脂滴包被蛋白和苹果酸酶。此外，运动训练改变的与防御和应激反应相关的基因表达，包括金属硫蛋白1和2以及热休克蛋白，显示出有趣的结果。我们的研究表明运动训练对预防饮食诱导肥胖小鼠的肥胖和代谢紊乱发展具有有益作用。
