Pedersen J T, Kaplan H, Wedeck L, Murphy R B, LoBue J, Pincus M R
Hematology Section, Veterans Administration Medical Center, NY.
J Lab Clin Med. 1990 Oct;116(4):527-34.
Human red blood cells with phenotype N/N and M/M were tested in an agglutination assay with anti-N and anti-M antibodies, respectively. After incubation of the synthesized octapeptide (Leu-Ser-Thr-Thr-Glu-Val-Ala-Met) from the N-amino terminus of glycophorin A, with anti-N antibody, there was significant inhibition of the agglutination of the N-positive cells. There was also inhibition of the agglutination of the M-positive cells with anti-M antibody by the synthesized octapeptide (Ser-Ser-Thr-Thr-Gly-Val-Ala-Met) from the M-amino terminus of glycophorin A. There was no inhibition, however, of the agglutination of M-positive cells with anti-M antibodies by the N-amino terminal octapeptide. Likewise, the M-amino terminal octapeptide did not inhibit agglutination of N-positive cells with anti-N. Because the synthesized octapeptides contained no carbohydrate, the anti-N and anti-M specificity appears to be determined principally by the peptides themselves. Further studies with the use of chimeric peptides indicate that the amino terminal amino acid leucine of N-glycophorin A is a primary determinant of the N antigen, whereas the amino terminal serine of M-glycophorin A is a primary determinant for the M antigen.
分别用抗 -N 和抗 -M 抗体对 N/N 和 M/M 表型的人类红细胞进行凝集试验。将从血型糖蛋白 A 的 N 端合成的八肽（Leu - Ser - Thr - Thr - Glu - Val - Ala - Met）与抗 -N 抗体孵育后，N 阳性细胞的凝集受到显著抑制。从血型糖蛋白 A 的 M 端合成的八肽（Ser - Ser - Thr - Thr - Gly - Val - Ala - Met）与抗 -M 抗体孵育后，M 阳性细胞的凝集也受到抑制。然而，N 端八肽对 M 阳性细胞与抗 -M 抗体的凝集没有抑制作用。同样，M 端八肽也不抑制 N 阳性细胞与抗 -N 的凝集。由于合成的八肽不含碳水化合物，抗 -N 和抗 -M 的特异性似乎主要由肽本身决定。使用嵌合肽的进一步研究表明，N - 血型糖蛋白 A 的 N 端氨基酸亮氨酸是 N 抗原的主要决定因素，而 M - 血型糖蛋白 A 的 N 端丝氨酸是 M 抗原的主要决定因素。
