Wu Chih-Yu, Wang Chung-Hsiung
Department of Entomology, National Taiwan University, Taipei, Taiwan, ROC.
J Invertebr Pathol. 2006 Nov;93(3):186-91. doi: 10.1016/j.jip.2006.08.003. Epub 2006 Sep 20.
Four new cell lines, designated as NTU-LY-1 to -4, respectively, were established from the pupal tissues of Lymantria xylina Swinhoe (Lepidoptera: Lymantriidae). These cell lines have been cultured approximately 80 passages during 2 years in TNM-FH medium supplemented with 8% fetal bovine serum, at a constant temperature of 28 degrees C. Each line consists of three major morphological types: round cells, spindle-shaped cells, and giant cells. The characterization of these cell lines showed that they are different from previously established lines derived from related Lepidopteran species. All new lines were susceptible to the L. xylina multiple nucleopolyhedrovirus (LyxyMNPV) and appeared to have a good potential for studying this virus.
分别从木毒蛾（鳞翅目：毒蛾科）的蛹组织中建立了四种新的细胞系，命名为NTU-LY-1至-4。这些细胞系在添加8%胎牛血清的TNM-FH培养基中于28摄氏度恒温下培养了约2年，传代约80次。每个细胞系由三种主要形态类型组成：圆形细胞、纺锤形细胞和巨型细胞。这些细胞系的特性表明它们与先前从相关鳞翅目物种建立的细胞系不同。所有新细胞系都对木毒蛾多核多角体病毒（LyxyMNPV）敏感，似乎具有研究这种病毒的良好潜力。
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