Department of Zoology, National Taiwan University, Taipei, Taiwan.
BMC Genomics. 2010 Feb 18;11:116. doi: 10.1186/1471-2164-11-116.
Outbreaks of the casuarina moth, Lymantria xylina Swinehoe (Lepidoptera: Lymantriidae), which is a very important forest pest in Taiwan, have occurred every five to 10 years. This moth has expanded its range of host plants to include more than 65 species of broadleaf trees. LyxyMNPV (L. xylina multiple nucleopolyhedrovirus) is highly virulent to the casuarina moth and has been investigated as a possible biopesticide for controlling this moth. LdMNPV-like virus has also been isolated from Lymantria xylina larvae but LyxyMNPV was more virulent than LdMNPV-like virus both in NTU-LY and IPLB-LD-652Y cell lines. To better understand LyxyMNPV, the nucleotide sequence of the LyxyMNPV DNA genome was determined and analysed.
The genome of LyxyMNPV consists of 156,344 bases, has a G+C content of 53.4% and contains 157 putative open reading frames (ORFs). The gene content and gene order of LyxyMNPV were similar to those of LdMNPV, with 151 ORFs identified as homologous to those reported in the LdMNPV genome. Two genes (Lyxy49 and Lyxy123) were homologous to other baculoviruses, and four unique LyxyMNPV ORFs (Lyxy11, Lyxy19, Lyxy130 and Lyxy131) were identified in the LyxyMNPV genome, including a gag-like gene that was not reported in baculoviruses. LdMNPV contains 23 ORFs that are absent in LyxyMNPV. Readily identifiable homologues of the gene host range factor-1 (hrf-1), which appears to be involved in the susceptibility of L. dispar to NPV infection, were not present in LyxyMNPV. Additionally, two putative odv-e27 homologues were identified in LyxyMNPV. The LyxyMNPV genome encoded 14 bro genes compared with 16 in LdMNPV, which occupied more than 8% of the LyxyMNPV genome. Thirteen homologous regions (hrs) were identified containing 48 repeated sequences composed of 30-bp imperfect palindromes. However, they differed in the relative positions, number of repeats and orientation in the genome compared to LdMNPV.
The gene parity plot analysis, percent identity of the gene homologues and a phylogenetic analysis suggested that LyxyMNPV is a Group II NPV that is most closely related to LdMNPV but with a highly distinct genomic organisation.
台湾重要的森林害虫木麻黄褐卷蛾每五到十年就会爆发一次。这种飞蛾的宿主植物范围已经扩大到包括 65 多种阔叶树。LyxyMNPV（木麻黄褐卷蛾多粒包埋型核型多角体病毒）对木麻黄褐卷蛾具有高度毒性，已被研究作为控制这种飞蛾的一种可能的生物农药。LdMNPV-like virus 也已从木麻黄褐卷蛾幼虫中分离出来，但 LyxyMNPV 在 NTU-LY 和 IPLB-LD-652Y 细胞系中的毒力均高于 LdMNPV-like virus。为了更好地了解 LyxyMNPV，测定并分析了 LyxyMNPV DNA 基因组的核苷酸序列。
LyxyMNPV 的基因组由 156344 个碱基组成，G+C 含量为 53.4%，包含 157 个推定的开放阅读框（ORF）。LyxyMNPV 的基因含量和基因顺序与 LdMNPV 相似，鉴定出 151 个 ORF 与 LdMNPV 基因组中报告的同源。两个基因（Lyxy49 和 Lyxy123）与其他杆状病毒同源，LyxyMNPV 基因组中鉴定出四个独特的 LyxyMNPV ORF（Lyxy11、Lyxy19、Lyxy130 和 Lyxy131），包括杆状病毒中未报告的 gag-like 基因。LdMNPV 包含 23 个在 LyxyMNPV 中不存在的 ORF。在 LyxyMNPV 中未发现似乎参与 L.dispar 对 NPV 感染易感性的基因宿主范围因子-1（hrf-1）的易识别同源物。此外，在 LyxyMNPV 中鉴定出两个推定的 odv-e27 同源物。与 LdMNPV 中的 16 个相比，LyxyMNPV 编码 14 个 bro 基因，占 LyxyMNPV 基因组的 8%以上。鉴定出 13 个同源区域（hrs），包含由 30 个碱基的不完全回文组成的 48 个重复序列。然而，与 LdMNPV 相比，它们在基因组中的相对位置、重复次数和方向上存在差异。
基因奇偶校验图分析、基因同源物的百分比同一性和系统发育分析表明，LyxyMNPV 是一种与 LdMNPV 密切相关的 II 组 NPV，但基因组组织高度不同。
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