Routtenberg A, Ehrlich Y H
Brain Res. 1975 Jul 18;92(3):415-30. doi: 10.1016/0006-8993(75)90326-1.
Endogenous phosphorylation of 4 membrane-bound protein components of cerebral cortex was studied in vitro. Cyclic AMP was shown to stimulate phosphorylation to a major extent in protein components designated D, E and G, but to a minor extent in protein component F. The time course of phosphorylation of each of these components is affected differently by (a) the presence of cyclic nucleotides or zinc ions and (b) the concentration of ATP and magnesium ions in the reaction mixture. Although a different membrane preparation was used here than by Ueda et al., molecular weight determination and response to divalent cations, magnesium and zinc, suggest that components D and E may be the same as their proteins I and II. The newly described phosphoproteins, F and G, are respectively the least and most stimulated by the addition of cyclic AMP. Under certain conditions phosphate content in protein E can be increased by cyclic AMP and decreased by cyclic GMP.23Author
对大脑皮层4种膜结合蛋白成分的内源性磷酸化进行了体外研究。结果表明，环磷酸腺苷（cAMP）在很大程度上刺激了指定为D、E和G的蛋白成分的磷酸化，但对蛋白成分F的刺激程度较小。（a）环核苷酸或锌离子的存在以及（b）反应混合物中ATP和镁离子的浓度对这些成分中每一种的磷酸化时间进程有不同影响。尽管此处使用的膜制剂与上田等人的不同，但分子量测定以及对二价阳离子镁和锌的反应表明，成分D和E可能与他们的蛋白I和II相同。新描述的磷蛋白F和G分别受到cAMP添加的最少和最多刺激。在某些条件下，蛋白E中的磷酸盐含量可因cAMP而增加，因环磷酸鸟苷（cGMP）而降低。23作者
