Kaplow J M, Bellot F, Crumley G, Dionne C A, Jaye M
Rhône-Poulenc Rorer Central Research, King of Prussia, PA 19406.
Biochem Biophys Res Commun. 1990 Oct 15;172(1):107-12. doi: 10.1016/s0006-291x(05)80179-2.
Heparin potentiates the mitogenic activity of acidic fibroblast growth factor (aFGF) by 20-100 fold but mechanisms detailing this potentiation have not yet been fully elucidated. We report that heparin increases the binding affinity of aFGF for the two cloned and overexpressed human FGF receptors, flg and bek, by 2-3 fold. This increase in binding affinity, together with previous data demonstrating a 3-5 fold increase in the stability of aFGF, are likely to account for a significant portion of heparin's potentiation of aFGF activity observed in biological assay systems.
肝素可使酸性成纤维细胞生长因子（aFGF）的促有丝分裂活性增强20至100倍，但这种增强作用的详细机制尚未完全阐明。我们报告称，肝素可使aFGF与两种克隆并过表达的人FGF受体flg和bek的结合亲和力提高2至3倍。结合亲和力的这种提高，连同先前的数据表明aFGF的稳定性提高了3至5倍，可能是在生物测定系统中观察到的肝素对aFGF活性增强作用的很大一部分原因。
