Mansukhani A, Dell'Era P, Moscatelli D, Kornbluth S, Hanafusa H, Basilico C
Department of Microbiology, New York University School of Medicine, NY 10016.
Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3305-9. doi: 10.1073/pnas.89.8.3305.
The bek gene encodes a member of the high-affinity fibroblast growth factor receptor family. The BEK/FGFR-2 receptor is a membrane-spanning tyrosine kinase with the typical features of FGF receptors. We have cloned a murine bek cDNA and expressed it in receptor-negative Chinese hamster ovary cells and in 32D myeloid cells. The BEK receptor expressed in Chinese hamster ovary cells binds acidic FGF, basic FGF, and Kaposi FGF equally well but does not bind keratinocyte growth factor or FGF-5 appreciably. Upon treatment with basic FGF or Kaposi FGF, the BEK receptor is phosphorylated and a mitogenic response is achieved. Heparan sulfate proteoglycans have been shown to play an obligate role in basic FGF binding to the high-affinity FLG receptor. Unlike the BEK-expressing Chinese hamster ovary cells, 32D cells expressing the BEK receptor require the addition of exogenous heparin in order to grow in the presence of basic FGF or Kaposi FGF. We show that the addition of heparin greatly enhances the binding of radio-labeled basic FGF to the receptor. Thus the BEK receptor, like FLG, also requires an interaction with heparan sulfate proteoglycans to facilitate binding to its ligands.
bek基因编码高亲和力成纤维细胞生长因子受体家族的一个成员。BEK/FGFR - 2受体是一种具有FGF受体典型特征的跨膜酪氨酸激酶。我们克隆了小鼠bek cDNA，并在受体阴性的中国仓鼠卵巢细胞和32D髓样细胞中进行了表达。在中国仓鼠卵巢细胞中表达的BEK受体对酸性FGF、碱性FGF和卡波西FGF的结合能力相同，但对角质形成细胞生长因子或FGF - 5的结合能力不明显。在用碱性FGF或卡波西FGF处理后，BEK受体发生磷酸化并产生促有丝分裂反应。硫酸乙酰肝素蛋白聚糖已被证明在碱性FGF与高亲和力FLG受体的结合中起重要作用。与表达BEK的中国仓鼠卵巢细胞不同，表达BEK受体的32D细胞在碱性FGF或卡波西FGF存在下生长需要添加外源性肝素。我们发现添加肝素可大大增强放射性标记的碱性FGF与受体的结合。因此，BEK受体与FLG一样，也需要与硫酸乙酰肝素蛋白聚糖相互作用以促进其与配体的结合。
