Ono K, Shioya H, Hakozaki M, Honda K, Mori T, Kochi H
Department of Biochemistry, Fukushima Medical College, Japan.
Biochem Biophys Res Commun. 1990 Oct 15;172(1):243-8. doi: 10.1016/s0006-291x(05)80200-1.
The activity of branched-chain 2-oxo acid dehydrogenase complex increased 3.0-fold in liver of rats fed on 0.1%(w/w) clofibrate. Immunotitration experiments with antibodies against the constituent enzymes of the complex revealed that this increase resulted mainly from the increased amounts of only two(a decarboxylase and a lipoate acyltransferase) of three components of the complex and that the other component(dihydrolipoamide dehydrogenase) remained unchanged in its content, irrespective of clofibrate administration. The increases of both enzyme components were associated with increases in their mRNA levels which were estimated by in vitro translation with poly(A)+ RNA.
