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Use of molecular cloning methods to map the distribution of epitopes on topoisomerase I (Scl-70) recognized by sera of scleroderma patients.

作者信息

D'Arpa P, White-Cooper H, Cleveland D W, Rothfield N F, Earnshaw W C

机构信息

Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

出版信息

Arthritis Rheum. 1990 Oct;33(10):1501-11. doi: 10.1002/art.1780331007.

DOI:10.1002/art.1780331007
PMID:1699543
Abstract

We report the initial molecular characterization of the autoimmune response against DNA topoisomerase I (topo I; Scl-70). Sera from 36 patients with scleroderma and 4 healthy control subjects were studied using 6 subcloned portions of topo I. Twenty-three sera recognized at least 2 independent epitopes on the molecule. Therefore, anti-topo I, like other non-organ-specific autoantibodies characterized to date, is polyclonal and multifocal. The cloned protein should prove suitable for sensitive early detection of anti-topo I in the clinical setting.

摘要

相似文献

1
Use of molecular cloning methods to map the distribution of epitopes on topoisomerase I (Scl-70) recognized by sera of scleroderma patients.
Arthritis Rheum. 1990 Oct;33(10):1501-11. doi: 10.1002/art.1780331007.
2
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引用本文的文献

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Immunol Rev. 2024 Nov;328(1):265-282. doi: 10.1111/imr.13390. Epub 2024 Sep 9.
2
IgM, IgG, and IgA anti-DNA topoisomerase I antibodies in systemic sclerosis.抗 DNA 拓扑异构酶 I 抗体 IgM、IgG 和 IgA 在系统性硬化症中的作用。
J Clin Lab Anal. 2009;23(6):408-16. doi: 10.1002/jcla.20342.
3
Analysis of human antitopoisomerase-I idiotypes.人抗拓扑异构酶-I独特型分析。
J Clin Invest. 1993 Sep;92(3):1302-13. doi: 10.1172/JCI116704.
4
The predominant form of mammalian DNA topoisomerase I in vivo has a molecular mass of 100 kDa.哺乳动物DNA拓扑异构酶I在体内的主要形式分子量为100千道尔顿。
Mol Biol Rep. 1994 Mar;19(2):99-103. doi: 10.1007/BF00997154.
5
Antitopoisomerase and anticentromere antibodies in the sclerodermatosus complex.硬皮病样综合征中的抗拓扑异构酶和抗着丝粒抗体。
Clin Rev Allergy. 1994 Fall;12(3):221-35. doi: 10.1007/BF02802319.
6
Detection of anti-topoisomerase I antibodies using a full length human topoisomerase I recombinant protein purified from a baculovirus expression system.使用从杆状病毒表达系统纯化的全长人拓扑异构酶I重组蛋白检测抗拓扑异构酶I抗体。
Clin Exp Immunol. 1995 May;100(2):214-8. doi: 10.1111/j.1365-2249.1995.tb03655.x.
7
The HLA-DR and DQ genes control the autoimmune response to DNA topoisomerase I in systemic sclerosis (scleroderma).HLA - DR和DQ基因控制系统性硬化症（硬皮病）中针对DNA拓扑异构酶I的自身免疫反应。
J Clin Invest. 1993 Sep;92(3):1296-301. doi: 10.1172/JCI116703.
8
B-cell epitopes of scleroderma-specific autoantigens.
Mol Biol Rep. 1992 Jun;16(3):183-9. doi: 10.1007/BF00464706.
9
Antitopoisomerase I monoclonal autoantibodies from scleroderma patients and tight skin mouse interact with similar epitopes.来自硬皮病患者和紧皮小鼠的抗拓扑异构酶I单克隆自身抗体与相似的表位相互作用。
J Exp Med. 1992 Apr 1;175(4):1103-9. doi: 10.1084/jem.175.4.1103.
10
Analysis of an immunodominant epitope of topoisomerase I in patients with systemic sclerosis.系统性硬化症患者拓扑异构酶I免疫显性表位的分析
Mol Biol Rep. 1992 May;16(2):117-23. doi: 10.1007/BF00419757.