A激酶锚定蛋白及相关环磷酸腺苷依赖性蛋白激酶在哺乳动物线粒体内腔的存在情况。
Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria.
作者信息
Sardanelli Anna Maria, Signorile Anna, Nuzzi Rosanna, Rasmo Domenico De, Technikova-Dobrova Zuzana, Drahota Zdenek, Occhiello Antonella, Pica Alessandra, Papa Sergio
机构信息
Department of Medical Biochemistry, Biology and Physics, University of Bari, Policlinico, Piazza Giulio Cesare 11, 70124 Bari, Italy.
出版信息
FEBS Lett. 2006 Oct 16;580(24):5690-6. doi: 10.1016/j.febslet.2006.09.020. Epub 2006 Sep 18.
Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by (32)P-phosphorylated C-PKA and of AKAP by (32)P-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody.
本文提供了证据，证明大鼠心脏线粒体内部存在AKAP121及相关的蛋白激酶A（PKA）。免疫印迹分析和胰蛋白酶消化模式表明，无论是从分离的线粒体还是心肌细胞培养物中制备线粒体时，90%或更多的线粒体C-PKA、R-PKA和AKAP121都定位于线粒体内腔。通过测量PKA的比催化活性、用（32）P磷酸化的C-PKA对R-PKA进行放射性标记、用（32）P磷酸化的R-PKA对AKAP进行放射性标记以及对暴露于金偶联的AKAP121抗体的线粒体进行电子显微镜观察，验证了这种定位。
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