Peña-Alvarez Araceli, Capella Santiago, Juárez Rocío, Labastida Carmen
Departamento de Química Analítica, Facultad de Química, Universidad Nacional Autónoma de México, Circuito Interior Cd. Universitaria, 04510 México D.F., Mexico.
J Chromatogr A. 2006 Nov 17;1134(1-2):291-7. doi: 10.1016/j.chroma.2006.09.005. Epub 2006 Sep 25.
Solid phase microextraction and capillary gas chromatography-mass spectrometry were used for the determination of seven terpenes in tequila. The method was selected based on the following parameters: coating selection (PA, PDMS, CW/DVB, and PDMS/DVB), extraction temperature, addition of salt, and extraction time profile. The extraction conditions were: PDMS/DVB fiber, Headspace, 100% NaCl, 25 degrees C extraction temperature, 30 min extraction time and stirring at 1200 rpm. The calibration curves (50-1000 ng/ml) for the terpenes followed linear relationships with correlation coefficients (r) greater than 0.99, except for trans,trans-farnesol (r = 0.98). RSD values were smaller than 10% confirmed that the technique was precise. Samples from 18 different trade brands of "Aged" tequila analyzed with the developed method showed the same terpenes in different concentrations. The analytical procedure used is selective, robust (more than 100 analyses with the same fiber), fast and of low-cost.
采用固相微萃取和毛细管气相色谱 - 质谱联用技术测定龙舌兰酒中的七种萜烯。该方法基于以下参数进行选择：涂层选择（PA、PDMS、CW/DVB和PDMS/DVB）、萃取温度、盐的添加以及萃取时间曲线。萃取条件为：PDMS/DVB纤维、顶空模式、100%氯化钠、25℃萃取温度、30分钟萃取时间以及1200转/分钟的搅拌速度。萜烯的校准曲线（50 - 1000 ng/ml）呈线性关系，相关系数（r）大于0.99，但反式，反式 - 金合欢醇（r = 0.98）除外。相对标准偏差（RSD）值小于10%，证实该技术具有精密度。用所开发的方法分析了18个不同商业品牌的“陈酿”龙舌兰酒样品，结果表明相同的萜烯存在于不同浓度的样品中。所采用的分析方法具有选择性、稳健性（同一根纤维可进行100多次分析）、快速且成本低的特点。
