Zhang Wei, Zeng Yuan-Shan, Zhang Xue-Bao, Wang Jun-Mei, Zhang Wei, Chen Shui-Jun
Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University at Guangzhou, Guangzhou, 510080, PR China.
Neurosci Lett. 2006 Nov 13;408(2):98-103. doi: 10.1016/j.neulet.2006.08.079. Epub 2006 Sep 25.
This study aims to investigate the effect of adenoviral vector-mediated neurotrophine-3 (NT-3) gene transfer and retinoic acid (RA) pretreatment on inducing neuronal differentiation of bone marrow mesenchymal stem cells (MSCs) in vitro. MSCs could be efficiently transduced by NT-3 gene via recombinant adenoviral vectors (Adv). Combination of AdvNT-3 and RA significantly promoted MSCs to differentiate into cell types associated with phenotypes of neural lineages, which included neural markers nestin, NF, MAP2 and PSD95 as detected by immunocytochemistry. But the expressions of GFAP in these cells were not obvious. RT-PCR analysis revealed that AdvNT-3 in combination with RA pretreatment could initiate the transcription of TrkC mRNA. These results demonstrate that the combination of AdvNT-3 and RA pretreatment may promote neuronal differentiation of MSCs, which may serve as ideal seed cells for the repair of spinal cord injury.
本研究旨在探讨腺病毒载体介导的神经营养因子-3（NT-3）基因转染及视黄酸（RA）预处理对体外诱导骨髓间充质干细胞（MSCs）向神经元分化的影响。通过重组腺病毒载体（Adv），NT-3基因可有效转导至MSCs。AdvNT-3与RA联合应用显著促进MSCs分化为与神经谱系表型相关的细胞类型，免疫细胞化学检测显示这些细胞表达神经标志物巢蛋白、神经丝蛋白、微管相关蛋白2（MAP2）和突触后致密物95（PSD95）。但这些细胞中胶质纤维酸性蛋白（GFAP）的表达不明显。逆转录聚合酶链反应（RT-PCR）分析显示，AdvNT-3与RA预处理联合应用可启动TrkC mRNA的转录。这些结果表明，AdvNT-3与RA预处理联合应用可能促进MSCs向神经元分化，这可能使其成为修复脊髓损伤的理想种子细胞。
