可逆蛋白质变性动力学。对海兔肌红蛋白的一项研究。

Kinetics of reversible protein denaturation. A study on aplysia myoglobin.

作者信息

Giacometti G M, Antonini E, Brunori M

机构信息

CNR Center of Molecular Biology and Institute of Chemistry, Faculty of Medicine, University of Rome, Rome, Italy.

出版信息

Biophys Chem. 1979 Jul;10(1):119-27. doi: 10.1016/0301-4622(79)80012-5.

DOI:10.1016/0301-4622(79)80012-5

PMID:16997212

Abstract

The kinetics of denaturation and renaturation of Aplysia Myoglobin by temperature-jump and stopped-flow are reported. The time course of the unfolding and refolding reveals the presence of significant amounts of intermediates both in absence and in the presence of alcohols. A linear three states reaction mechanism is proposed and discussed.

摘要

报道了利用温度跃升和停流技术研究海兔肌红蛋白变性和复性的动力学。展开和重折叠的时间进程表明，无论有无醇类存在，都有大量中间体存在。提出并讨论了一个线性三态反应机制。