Jordan P C, Stark G
Fakultät fur Biologie. Universität Konstanz. D-7750 Konstanz, G.F.R.
Biophys Chem. 1979 Nov;10(3-4):273-87. doi: 10.1016/0301-4622(79)85016-4.
Previous interpretations of the kinetics of transport of hydrophobic ions through membranes have been based on one of three limiting assumptions. Either diffusion in the aqueous phase was taken to be rapid, or ionic motion was constrained to the membrane or a steady state was presumed to be established within the membrane. We present a general treatment of the coupled diffusion process through both the aqueous phase and the membrane; our theory contains the previous results as limiting cases. It is applied to voltage jump-current relaxation experiments on black lipid membranes in the presence of dipicrylamine or sodium tetraphenylborate. We have attempted to establish the rate of desorption from the membrane. For the system phosphatidylserine/tetraphenylborate, the rate of desorption and the rate of translocation were found to be comparable.
以往对疏水性离子跨膜传输动力学的解释基于三个极限假设之一。要么认为在水相中的扩散很快，要么离子运动局限于膜内，要么假定在膜内建立了稳态。我们给出了通过水相和膜的耦合扩散过程的一般处理方法；我们的理论包含了以前的结果作为极限情况。它被应用于在存在二硝基苯胺或四苯硼酸钠的情况下对黑色脂质膜进行的电压阶跃 - 电流弛豫实验。我们试图确定从膜上的解吸速率。对于磷脂酰丝氨酸/四苯硼酸盐体系，发现解吸速率和转运速率相当。
