Vanhaecke Mieke, Van den Ende Wim, Van Laere André, Herdewijn Piet, Lescrinier Eveline
Laboratory of Molecular Plant Physiology, Institute of Botany and Microbiology, K.U. Leuven, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium.
Carbohydr Res. 2006 Nov 27;341(16):2744-50. doi: 10.1016/j.carres.2006.09.001. Epub 2006 Sep 25.
Lychnose (alpha-D-Gal-(1-->6)-alpha-D-Glc-(1-->2)-beta-D-Fru-(1-->1)-alpha-D-Gal) was isolated from Stellaria media, a representative member of the Caryophyllaceae plant family. Weak acid hydrolysis, enzymatic hydrolysis and complete NMR characterization were performed to confirm the identity of the tetrasaccharide. All (1)H and (13)C resonances were unambiguously assigned and the conformation of the sugars was determined using one and two dimensional NMR techniques. Anomeric characterizations in lychnose were confirmed from HMBC and NOESY spectra.
棉子糖（α-D-半乳糖-（1→6）-α-D-葡萄糖-（1→2）-β-D-果糖-（1→1）-α-D-半乳糖）是从石竹科植物家族的代表性成员繁缕中分离得到的。通过弱酸水解、酶水解和完整的核磁共振表征来确认四糖的结构。对所有的¹H和¹³C共振信号进行了明确归属，并使用一维和二维核磁共振技术确定了糖的构象。通过HMBC和NOESY谱确认了棉子糖中的端基特征。
