Lim A T, Dean B, Copolov D
Neuroendocrine Laboratory, Mental Health Research Institute of Victoria, Royal Park Hospital, Parkville, Australia.
Endocrinology. 1990 Nov;127(5):2598-600. doi: 10.1210/endo-127-5-2598.
In rats, the precursor of atrial natriuretic peptide (ANP) is produced and processed into its smaller congeners in the heart and the brain. We have demonstrated that a congener of ANP, auriculin B (ANP4-28), was present in long term monolayer cultures of neonatal rat hypothalamic neurons. In addition, forskolin and 3-isobutyl-1-methyl-xanthine (IBMX) augmented the cellular content of auriculin B in a dose dependent manner. Thus, cyclic AMP may act as an intracellular signal for modulating the functional development of hypothalamic immunoreactive (ir) ANP producing neurons. Furthermore, our data suggest that the form of ANP released from these cultures, following either forskolin treatment alone or forskolin treatment followed by acute high potassium depolarisation, was atriopeptin III (ANP5-28). Thus atriopeptin III, rather than auriculin B, may represent the endogenous ligand for ANP receptors in the central nervous system.
在大鼠中，心房利钠肽（ANP）的前体在心脏和大脑中产生并加工成其较小的同系物。我们已经证明，ANP的一种同系物耳房素B（ANP4-28）存在于新生大鼠下丘脑神经元的长期单层培养物中。此外，福斯可林和3-异丁基-1-甲基黄嘌呤（IBMX）以剂量依赖性方式增加了耳房素B的细胞含量。因此，环磷酸腺苷（cAMP）可能作为一种细胞内信号，用于调节下丘脑免疫反应性（ir）ANP产生神经元的功能发育。此外，我们的数据表明，在单独使用福斯可林处理或福斯可林处理后进行急性高钾去极化后，这些培养物释放的ANP形式是心钠素III（ANP5-28）。因此，心钠素III而非耳房素B可能代表中枢神经系统中ANP受体的内源性配体。
